STAT1、STAT6在支气管哮喘小鼠中的表达及地塞米松对其表达的影响  

Expression of STAT1,STAT6 in murine bronchial asthma model and effect of dexamethasone on the expression

作　　者：郑美梅[1] 王方剑[1] 段成城[1] 

机构地区：[1]广州医学院附属深圳沙井医院呼吸内科,518104

出　　处：《中华哮喘杂志（电子版）》2010年第1期4-6,共3页Chinese Journal of Asthma(Electronic Version)

摘　　要：目的研究信号转导和转录激活因子1(signal transducer and activator of transcription1,STAT1)、信号转导和转录激活因子6(STAT6)在支气管哮喘(简称哮喘)气道炎症中的作用和地塞米松对其表达的干预。方法雄性BALB/c小鼠随机分为对照组(A组)、哮喘组(B组)和地塞米松组(C组),每组8只。逆转录-聚合酶链反应(RT-PCR)法半定量测定肺组织中STAT1、STAT6mRNA的表达水平,组织学观察肺组织炎症程度。结果①B组肺组织中STAT1mRNA表达水平显著高于A组(P<0.01),而C组显著低于B组(P<0.01);②B组肺组织中STAT6mRNA表达水平显著高于A组(P<0.01),而C组显著低于B组(P<0.01);③C组小鼠肺组织中STAT1mRNA与STAT6mRNA的表达呈显著正相关(r=0.939,P<0.01)。结论 STAT1、STAT6的高表达参与了哮喘小鼠的病理生理过程;地塞米松可同时下调STAT1、STAT6的表达,抑制哮喘气道炎症。Objective To investigate the effects of signal transducer and activator of transcription 1 （STAT1）.STAT6 in ovalbumin challenged asthmatic mice and observe the change of STAT1. STAT6 interfered by dexamethasone. Methods BALB/e male mice were randomly divided into 3 groups： control group,asthma group,and dexamethasone group, with 8 mice in each group. The expressions of STAT1. STAT6 mRNA in lung tissue were detected by RT PCR. The pathological changes of lung were measured by HE staining. Results Compared with asthmatlc group,the expression of STAT1 ,STAT6 mRNA were lower significantly（ P 〈0.01） in control group and dexamethasone group. STAT1 mRNA expression of lung tissue in dexamethasone group was correlated with STAT6 mRNA expression significantly （ r ： 0. 939, P 〈0.01）. Conclusions The high expression of STAT1 and STAT6 is involved in the pathophysiological process of mouce asthma development. Dexamethasone can down regulate the expression and inhibit the airway inflammation in asthma.

关 键 词：支气管哮喘 信号转导和转录激活因子 地塞米松 

分 类 号：R563[医药卫生—呼吸系统]