免疫印迹法检测肝自身抗体谱及其临床应用  

Detection of Liver Antoantibodies by Western blot and its clinical application

作　　者：陆兴热[1] 资云菊[1] 高仕萍[1] 谭锐[1] 和润泞[1] 袁雕[1] 陈凤羽[1] 刘孝文[1] 缪丽萍[1] 

机构地区：[1]文山州人民医院检验科,云南文山663000

出　　处：《中国中医药咨讯》2010年第7期99-100,共2页

摘　　要：目的：寻找自身免疫性肝脏疾病自身抗体谱及其检测方法，并对此法的可靠性、准确性、临床价值、推广价值进行总结及评价。方法：应用免疫印迹技术检测202不明原因肝功能异常，用干扰素等病毒性肝炎的治疗方案无效的患者、69例自身免疫性肝病患者、60例慢性乙肝患者、20例丙型肝炎患者和30例到本院体检的健康体检者的自身抗体。结果：自身免疫性肝病组自身抗体检出的总阳性率分别为AIH92．8％、PBC93．9％、PSC87．5％。自身免疫性肝病组与病毒性肝炎组和正常对照组间差异有显著性意义（P〈0．01）。HBV组和HCV组自身抗体检出的总阳性率分别为6．7％、15.0％。AIH自身抗体谱特征主要表现为ANA、SMA、LKM-1、SLA／LP、LC-1阳性，特别是SLA／LP、LC-1只在AIH组中表现为阳性。PBC自身抗体谱特征主要表现为ANA、AMA—M2阳性。PSC自身抗体谱特征主要表现为ANA、ANCA阳性。202例不明原因肝功能异常患者中11例自身抗体谱阳，阳性率为5．4％。结论：免疫印迹法是检测自身免疫性肝脏疾病自身抗体的一种理想方法。Objective To Search for autoantibodies spectrum in autoimmune liver disease and to assess the reliability, accuracy, clinical value of this method. Methods 381 samples were tested using western blot including 202 cases of unexplained abnormal liver function, 69 cases of antoimmune liver disease, 60 patients with chronic hepatitis B patients, 20 cases of hepatitis C patients and 30 people to the hospital for health check-ups.Results In autoimmune liver disease group, AIH, PBC, PSC positive rate of each was 92.8%, 93.9%, 87.5%, The difference is significant among Autoimmune fiver diseases, viral hepatitis group and the normal control group （p〈0.01）. The Autoantibodies total positive rate of HBV group and HCV group each was 6.7% and 15.0%.The main features of AIH autoantibody spectrum showed that ANA, SMA, LKM-1, SLA / LP, and LC-1 are positive. Conclusion Western blot is an ideal method for detecting autoantihodies in autoimmune liver disease.

关 键 词：免疫印迹 自身免疫性肝病 自身抗体 

分 类 号：R593.241[医药卫生—内科学]