男女新生儿脐带血血清蛋白质组的比较  

The Comparison of Proteome between Male and Female Neonatal Umbilical Cord Serum

作　　者：宋红娟[1,2] 季晖[1] 刘嘉茵[1,2] 

机构地区：[1]中国药科大学,江苏南京210009 [2]南京医科大学第一附属医院临床医学生殖中心,江苏南京210036

出　　处：《药物生物技术》2010年第2期146-150,共5页Pharmaceutical Biotechnology

基　　金：国家基础研究项目(NO.2006CB944005;2006CB701503);国家863高科技基金项目(NO.2006AA02Z4A4);教育部创造力团队计划项目(NO.IRT0631);江苏省教育厅基金项目(NO.JHB06-13)

摘　　要：为发现不同性别新生儿之间蛋白质表达谱的差异,利用胰蛋白酶对上述血清蛋白分别进行酶解,并通过反相高效液相色谱法(RP-HPLC)对肽段混合物进行了分离,进一步通过电喷射离子化串联质谱法(ESI-MS/MS)鉴定了分离的肽段。本研究中共鉴定出837种非冗余蛋白质,其中不同类型血清中共有的蛋白质有282种,另有239种和213种分别属于男性新生儿和女性新生儿特有蛋白质。对其中53种差异蛋白质进行了分析,初步探讨了它们在胎儿生长发育以及性别分化过程中的作用。To find out the protein markers between male and female fetus, proteomic analyses were used in this study to compare the proteomes of male and female neonatal umbilical cord serum. The serum proteins were tryptic digested and separated by reverse-phase high-performance liquid chromatography. The fragmented peptide mixtures were further identified by electrospray ionization tandem mass spectrometry. Totally 837 non-redundant proteins were identified, among which 213 and 239 proteins were confirmed to be the special proteins in the neonatal umbilical cord serum of male and female fetus, respectively. However, at the same time, there were 282 proteins identified in all the tested serum samples. Fifty-three differentially displayed proteins were further analyzed to uncover the roles of them in the process of fetus develoDment and sex differentiation.

关 键 词：人脐带血清 蛋白质组 差异蛋白 

分 类 号：Q51[生物学—生物化学]