胆红素生产中的脱脂方法与酸化度研究  

Study on Acidification Degree and Defatting Method in Producing Bilirubin

作　　者：黄仁术[1] 

机构地区：[1]皖西学院化学与生命科学系,安徽六安237012

出　　处：《药物生物技术》2010年第2期162-164,共3页Pharmaceutical Biotechnology

基　　金：安徽省高等学校省级自然科学研究项目<獭兔胆汁的综合利用研究>(编号KJ2009B087)

摘　　要：为提高胆红素生产效益,采取正交试验优化筛选脱脂方法(双层纱布法、石油醚洗涤法、混合法)和酸化度(pH5.0、pH5.5、pH6.0),结果表明:脱脂方法和酸化度对胆红素提取差异高度显著(P<0.01),影响度脱脂方法>酸化度;在双层纱布过滤、酸化至pH5.0时萃取的常规条件下,胆红素的粗制品收率0.0923 g/L、纯度36.18%,折合纯品收率0.0333 g/L;在混合法脱脂、酸化至pH5.5时萃取的条件下,胆红素的粗制品收率0.6122 g/L、纯度52.05%,折合纯品收率最高,达0.3186 g/L。To improve the efficiency of producing bilirubin, orthogonal test was used in optimum screening methods （two-layer absorbent cotton method, petroleum ether method and the combind method） and acidification degree （pH 5.0, pH 5.5, pH 6.0）. The result has showed that the differences between different defatting methods and acidification degree in producing bilirubin are greatly significant （P〈0.01）, and the influence of defatting methods is greater than acidification degree. Extracted in common situation by two-layer absorbent cotton method and acidification degree of pH 5.0, the raw product yield of bilirubin is 0. 092 3 g/L, the purity is 36. 18% and the pure product yield is 0. 033 3 g/L. Extracted by combind method and acidification degree of pH 5.5, the raw product yield of bilirubin is 0. 612 2 g/L, the purity is 52.05% and the pure product yield is the highest, reaching 0. 318 6 g/L.

关 键 词：胆红素 快速法 脱脂方法 酸化度 

分 类 号：R282.74[医药卫生—中药学] TQ460.6[医药卫生—中医学]