重症急性胰腺炎肠微循环的改变与细菌移位关系的实验研究  

The relationship between microcirculation and bacterial translocation in severe acut pancreatitis

作　　者：陈建中[1,2] 戴植本[1,2] 

机构地区：[1]同济医科大学附属同济医院普外科 [2]汕头大学医学院附属第一医院普外科

出　　处：《中华外科杂志》1998年第A00期47-49,共3页Chinese Journal of Surgery

摘　　要：目的运用放射性生物微球技术测定肠血流量，了解重症急性胰腺炎肠血流量的改变与细菌移位的关系。方法３０只大鼠随机分成三组，每组１０只。Ⅰ组：血流实验组。Ⅱ组：细菌移位实验组。Ⅲ组：正常血流对照组。第Ⅰ组在诱导胰腺炎２５小时后运用放射性生物微球技术测定肠血流量。第Ⅱ组在诱导胰腺炎２５小时后同时期收集肠系膜淋巴结、胰腺、腹水、肝脏作培养，并通过抗菌谱和质粒ＤＮＡ分析对携质粒大肠杆菌作鉴定。结果重症急性胰腺炎发病后２５小时，包括近端小肠、远端小肠和结肠在内的肠血流量都显著降低（Ｐ＜００１），但此时肠道基本无细菌移位发生。Objective To measure the blood flow of intestine and explore the relationship between the changes of blood flow in intestine of rats with severe acute pancreatitis and bacterial translocation. Method Thirty rats were randomly divided into three groups (each group 10 rats):experimental group of blood flow (Ⅰ); experimental group of bacterial translocation (Ⅱ);normal control group of blood flow (Ⅲ).Radioactive biomicrosphere technique, was used to measure the blood flow of intestinal segments for 2 5 hours after induction of severe acute pancreatitis in group Ⅰ. The mesenteric lymph node, pancreas, peritoneal fluid and liver were harvested for culture at the same period after induction in group Ⅱ,and E.Coli which bears plasmids was identified by antibiography and plasmid DNA analysis. Result The blood flow of intestinal segments, proximal small bowl,distal small bowl and colon was decreased significantly 2 5 hours after induction of severe acute pancreatitis( P <0 01),and no bacterial translocation occurred at the same time. Conclusion Macosal injury which led by the change of blood flow may be the primary cause why bacterial translocation happens.

关 键 词：胰腺炎 微循环 微球体 细菌移位 

分 类 号：R657.51[医药卫生—外科学]