滋阴益气活血组方对STZ糖尿病大鼠免疫器官影响  

Effects of yin-enriching, qi-boosting and blood-quickening compound formula on immune organs in diabetic rats induced by STZ

作　　者：李静[1] 刘德义[1] 程昌勇[1] 安好峰[1] 杨东星[1] 

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100

出　　处：《中兽医医药杂志》2010年第2期14-18,共5页Journal of Traditional Chinese Veterinary Medicine

基　　金：安徽科技学院引进人才专项基金资助项目(ZRC2007139)

摘　　要：目的:探讨自拟滋阴益气活血组方对链脲佐菌素(STZ)糖尿病大鼠免疫器官影响。方法:用STZ尾静脉注射45mg/kg诱导建立糖尿病大鼠模型,将符合糖尿病诊断标准的大鼠随机分为模型对照组(n=15)和组方治疗组(n=15),同时设正常对照组(n=20)。组方治疗组灌胃滋阴益气活血组方水煎液10mL/kg.d,模型对照组和正常对照组灌胃相同剂量的生理盐水,连续灌胃56d。灌胃第14、28、42、56天眶后静脉丛采血,制备血清以测定血糖(GLU)水平。第56天剖杀大鼠,取脾脏、胸腺组织,制作切片,观察组织结构。结果:第56天时,组方治疗组的血糖水平极显著低于模型对照组(P<0.01),基本接近正常水平;与模型对照组相比,组方治疗组可显著提高脾脏指数和胸腺指数;模型对照组脾小结数量较少,生发中心不够明显;组方治疗组脾小结数量体积较正常,可以见到生发中心;模型对照组胸腺组织结构变化明显,胸腺小体数量较少;治疗组胸腺小叶较大,胸腺小体较多。结论:自拟滋阴益气活血组方能够降低糖尿病大鼠的血糖,对STZ糖尿病大鼠的脾脏、胸腺及其组织结构损伤有一定的治疗作用。Purpose：The aim of the research is to study the effects of yin-enriching, qi-boosting and blood-quickening compound formula（YQBCF） on immune organs in diabetic rats induced by streptozotocin（STZ）. Methods： 45mg/kg STZ was injected through caudal vein to establish diabeticⅡ rat models, and the rats were randomly divided into YQBCF treatment group and model control group, then establish the normal control group （n=20） contemporarily. The treatment group was given YQBCF decoction at 10 mL/kg·d for 56 days by stomach perfusion, the model control group and the normal control group were given the same dose of distilled water. On 14th, 28th, 42nd and 56th, rat serum was collected to detect the contents of glucose（GLU）. On 56th, slices was made to observe the organizational structure of spleen and thymus. Results： On 56th, glucose concentration in YQBCF treatment group were significantly decreased compared with model control group（P0.01）, which nearly had tended to normal levels. Compared with the model control group, the spleen index and thymus index in treated group were significantly increased, had a normal number of splenic lymph nodules and thymic corpuscles, as well as visible germinal centers and larger thymus lobule. Conclusion： The data demonstrated that YQBCF have a certain therapeutic effect by reducing blood glucose and decreasing organizational structure damage in diabetic rats induced by STZ.

关 键 词：糖尿病 滋阴益气活血 链脲佐菌素 血糖 脾脏 胸腺 

分 类 号：S853.74[农业科学—临床兽医学]