正交试验法优选金银花中绿原酸超声波强化浸出工艺研究  

Optimization on the ultrasonic treatment extracting process for chlorogenic acid from Lonicera japonica Thunb. by orthogonal design

作　　者：宋德花[1] 梁运霞[1] 杨光宇[1] 张子群 谢晓峰 李继昌[3] 

机构地区：[1]黑龙江畜牧兽医职业学院,黑龙江双城150111 [2]黑龙江出入境检验检疫局 [3]东北农业大学动物医学院

出　　处：《中兽医医药杂志》2010年第2期37-40,共4页Journal of Traditional Chinese Veterinary Medicine

基　　金：黑龙江省科学技术厅科技攻关项目(20020101053)

摘　　要：目的:优选金银花中绿原酸超声波强化浸出工艺。方法:采用正交试验法,以测定金银花中绿原酸提取率作为考核指标。结果:金银花中绿原酸超声波强化浸出工艺的最佳提取条件为乙醇浓度70%,8倍量,超声浸出30min,绿原酸提取率高。结论:该试验绿原酸提取率高,稳定性好。Objective：To optimize the ultrasonic treatment extracting process for chlorogenic acid from Lonicera japonica Thunb.. Methods： Orthogonal design was used to determine the optimum extracting process with the extracting rate of chlorogenic acid as makers. Results： The ultrasonic treatment optimum extracting process for chlorogenic acid from Lonicera japonica Thunb. was that adding 8 folds of 70% ethanol, refluxing for 30 min. Conclusion： The extracting process for chlorogenic acid shows high extracting rate and good stability.

关 键 词：金银花 绿原酸 提取率 正交试验法 HPLC法 

分 类 号：S853.74[农业科学—临床兽医学]