吡喹酮对血吸虫卵肉芽肿与肝纤维化作用的研究Ⅱ吡喹酮对小鼠肺部血吸虫卵肉芽肿内细胞的影响  

Effect of praziquantel on schistosomal ovum granuloma and hepatic fibrosisⅡ Effect of praziquantel on cells within pulmonary schistosomal ovum granuloma in mice

作　　者：许永良[1] 李洪军[1] 黄一心[1] 余传信[1] 王铁生[2] 吴中兴[1] 

机构地区：[1]江苏省寄生虫病防治研究所,无锡214064 [2]江苏省江原医院

出　　处：《中国血吸虫病防治杂志》2010年第2期105-110,共6页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(30671833);江苏省科技厅公益专项(BM2007704);江苏省医学重点人才基金(RC2007095)

摘　　要：目的研究应用吡喹酮后小鼠肺部血吸虫卵肉芽肿内细胞的变化。方法48只小鼠均分4组。A组:虫卵致敏组,先于小鼠腹壁皮下注入新鲜日本血吸虫卵,10d后由尾静脉注入虫卵;B组:吡喹酮短期应用组,致敏方法同A组,于尾静脉注入虫卵前1d起灌服吡喹酮300mg/(kg.d)连续3d;C组:吡喹酮持续应用组,致敏方法同A组,于尾静脉注入虫卵前1d起灌服吡喹酮75mg/kg,1日2次,连续5d,停药2d后反复用药至剖检;D组:第29天起应用吡喹酮组,服法同C组。各组均在尾静脉注射虫卵后7、14、28、56d分别剖杀3只,取肺组织切片,HE染色,在每组肺连续切片内选含毛蚴的虫卵肉芽肿25～30个,分类计数每个肉芽肿内的中性粒细胞、嗜酸性粒细胞、淋巴细胞、纤维母细胞、巨噬细胞,计算其平均值并作比较。结果与A组比较,应用吡喹酮的3个实验组,中性粒细胞、嗜酸性粒细胞、巨噬细胞均显著减少,纤维母细胞增高的幅度也明显为低,差异均有统计学意义,而以C组最为显著,第56天,中性粒细胞、嗜酸性粒细胞、巨噬细胞分别减少了54.4%、87.0%和23.1%,纤维母细胞增高的幅度减少了59.4%。各组淋巴细胞数目变化不大。结论吡喹酮能明显抑制虫卵肉芽肿内炎细胞的聚集、纤维母细胞的增生。Objective To study the effect of praziquantel on cells within schistosomal ovum granuloma in the lung of mice.Methods Forty-eight mice were divided into 4 groups.Group A：first,the mice were injected with schistosomal ova hypodermically in abdomen,and 10 days later,injected with schistosomal ova intravenously in the cauda;Group B：in addition to the injection of schistosomal ova as the same of Group A,the mice were administered with praziquantel [300 mg/（kg·d）] for 3 days,one day before the intravenous injection of the ova;Group C：in addition to the injection of schistosomal ova as the same of Group A,the mice were administered with praziquantel （75 mg/kg,B.i.d.） for 5 days weekly until the mice were sacrificed;Group D：the same as Group C but praziquantel was given to the mice from the 29th after the intravenous injection of the ova.Three mice of each group were sacrificed on the 7th,14th,28th,56th day after the intravenous injection of the ova and the lung tissues were fixed with formalin and the slices were HE stained.Twenty-five to thirty pieces of schistosomal ovum granuloma were examined and the neutrophilic granulocytes,eosinocytes,lymphocytes,fibroblasts and macrophages within the ovum granulomas were counted and the mean numbers of them of each group were calculated and compared.Results Compared with Group A,the mean numbers of neutrophilic granulocytes,eosinocytes and macrophages within the ovum granulomas were decreased significantly,and the extend of the increase of fibroblasts reduced significantly in the three groups administered with praziquantel,and especially in Group C.On the 56th day after the intravenous injection of the ova,the mean numbers of neutrophilic granulocytes,eosinocytes and macrophages decreased by 54.4%、87.0% and 23.1%,and the extend of the increase of fibroblasts reduce by 59.4%,respectively in Group C,compared with Group A.The numbers of lymphocytes did not change very much in 4 groups.Conclusion Praziquantel can restrain inflammatory cells and the hyperplasia of

关 键 词：吡喹酮 日本血吸虫 肉芽肿 中性粒细胞 嗜酸性粒细胞 淋巴细胞 纤维母细胞 巨噬细胞 

分 类 号：R383.24[医药卫生—医学寄生虫学]