胶体染料试纸条法用于血吸虫病低度流行区筛查效度评价  

Validity evaluation of dipstick dye immuno-assay (DDIA) for screening in low endemic areas of schistosomiasis

作　　者：张世微[1] 程斌[2] 渠洪捷[2] 陈仲民[2] 邹琴[2] 褚丽平[2] 张琳 何焕然 汤胜华 黄小平 杨红梅 

机构地区：[1]湖北职业技术学院医学分院,孝感432000 [2]湖北省孝感市疾病预防控制中心 [3]湖北省孝感市血吸虫病防治研究所 [4]湖北省孝感市孝南区政府血吸虫病防治工作领导小组办公室

出　　处：《中国血吸虫病防治杂志》2010年第2期171-173,共3页Chinese Journal of Schistosomiasis Control

基　　金：湖北省卫生厅科研攻关重点项目并资助课题(XF06C14)

摘　　要：目的探索胶体染料试纸条法(DDIA)在孝感市血吸虫病低度流行区的应用价值。方法在孝感市低度流行区随机选择一自然村,对6～65岁村民同时采用粪便集卵孵化法、DDIA、IHA、ELISA和询检筛查同一群体,以粪便集卵孵化法为标准,评价其他几种方法的效度,并比较其费用。结果共筛查287人,粪检、IHA、DDIA、ELISA及询检阳性率分别为1.74%、7.67%、9.06%、19.51%、29.97%。以粪检为金标准,IHA、DDIA、ELISA及询检约登指数分别为0.74、0.72、0.62、0.30,符合率分别为93.38%、91.99%、81.53%、70.03%,上述方法筛查1例病人所需费用分别为16.70、4.95、4.12、5.63、2.44元。结论DDIA用于低度流行区血吸虫病筛查效度较好,且操作简便、费用经济,可用于大规模筛查。Objective To explore the application value of dipstick dye immuno-assay （DDIA） for screening the schistosomiasis chemotherapy targets in the low endemic areas of Xiaogan City.Methods The residents aged 6-65 years in a village in the low endemic areas of schistosomiasis of Xiaogan City were selected and tested by the methods of fecal examination,DDIA,indirect hemagglutination （IHA）,enzyme linked immunosorbent assay （ELISA） and inquiry,and the results of fecal examination were determined as the gold standard.Results The Youden’s indices of IHA,DDIA,ELISA and inquiry were 0.74,0.72,0.62 and 0.30,respectively,and the consistency rates of them were 93.38%,91.99%,81.53% and 70.03%,respectively.It took 16.70,4.95,4.12,5.63 and 2.44 Yuan screening one patient with the fecal examination,IHA,DDIA,ELISA and inquiry,respectively.Conclusion The validity of DDIA with simple operation and low cost for screening the schistosomiasis chemotherapy targets is satisfying,and the method is suitable for large scale screening in low endemic areas.

关 键 词：血吸虫病 筛查 效度 胶体染料试纸条法 低度流行区 

分 类 号：R532.21[医药卫生—内科学]