番茄种质遗传多样性的AFLP分析  

Genetic diversity of tomato by AFLP analysis

作　　者：姚祝平[1] 叶青静[1] 杨悦俭[1] 王荣青[1] 阮美颖[1] 周国治[1] 

机构地区：[1]浙江省农业科学院蔬菜研究所,浙江杭州310021

出　　处：《浙江农业学报》2010年第2期156-160,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省科技计划项目(2007D70009);农业科技成果转化资金项目(2007GB2C200129);浙江省科技厅重大科技专项(优先主题)农业项目(2008C120022)

摘　　要：利用AFLP技术对44份番茄材料进行遗传多样性和亲缘关系分析。从64对引物组合中筛选出15对用于扩增,共获得601条带,其中多态性带364条,多态性为60.5%,这表明供试材料在DNA水平上酶切位点的分布存在广泛的变异。利用UPGMA法将44份番茄材料分成5个复合组和1个独立组,从相似性系数分析了各材料之间的亲缘关系。The genetic diversity and relationship of 44 tomato accessions were analyzed by AFLP （amplified fragment length polymorphism）. Fifteen primer combinations selected from 64 primer combinations produced a total of 601 fragments, 364 of which were polymorphic with a polymorphism percentage of 60.5%. This results showed that there were abundant diversities in enzyme digestion sites among different tomato varieties. These tomato germplasm resources were clustered into 5 multiple-member groups and 1 one-member group by UPGMA （unweighted pair group method with arithmetic average） based on Nei＇s genetic distances. The genetic relationship of 44 tomato accessions was analyzed using the different coefficient.

关 键 词：番茄 AFLP 遗传多样性 亲缘关系 

分 类 号：S641.2[农业科学—蔬菜学]