一株浸矿细菌的分离及其对低品位硫化镍铜矿中镍的浸出  

Isolation of Strain Ynxd-1 and Its Leaching Efficiency of Low-Grade Sulfide Nickel and Copper Ore

作　　者：熊学权[1] 季秀玲[1] 魏云林[1] 林连兵[1] 

机构地区：[1]昆明理工大学生物工程技术研究中心,云南昆明650224

出　　处：《昆明理工大学学报（理工版）》2010年第2期20-26,共7页Journal of Kunming University of Science and Technology(Natural Science Edition)

基　　金：国家自然科学基金项目(项目编号:30660009);云南省自然科学基金项目(项目编号:2005E0068M)资助

摘　　要：从云南某铜矿井下酸性污水中分离到嗜酸菌株ynxd-1,对其形态、生长特性、16 S rRNA基因序列及其对低品位硫化镍铜矿的摇瓶浸出效果进行了研究.结果表明:细菌细胞呈短杆状,革兰氏染色阴性,不产芽孢,最适pH值及生长温度分别为2.0和30℃.菌株ynxd-1在10%(W/V)矿浆浓度及30℃温度的条件下摇瓶培养浸出28 d,低品位硫化镍铜矿镍的浸出率为56.4%.16 S rRNA基因序列分析表明,菌株ynxd-1与嗜酸氧化亚铁硫杆菌的同源性达到99%,可以鉴定为嗜酸氧化亚铁硫杆菌菌株.An acidophilic strain ynxd - 1 is isolated from an acid copper mine drainage, and its morphology, growth characteristics, 16S rRNA gene sequence, low -grade sulfide nickel and copper ore leaching efficiency are then studied. The result shows that the bacteria cell is rod - shaped, gram - negative and that spore formation is not observed. It grows optimally at 30℃ and pH 2.0. After leached with strain ynxd - 1 in shaken flask at 30℃ for 28 days, the leaching efficiency of low- grade sulfide nickel and copper ore pulp （10% , W/V） reaches 56.4%. On the basis of 16S rRNA gene sequence similarity, strain ynxd - 1 is shown to belong to AcidithiobaciUus ferrooxidans, being related to the type strains of A. ferrooxidans （99% similarity）.

关 键 词：生物浸出 低品位硫化镍铜矿 16S RRNA基因 氧化亚铁硫杆菌 

分 类 号：Q937.97[生物学—微生物学]