白腐菌Trametes hirsuta BYBF产漆酶发酵条件的优化  

Studies on Fermentation Conditions for Laccase Production from White-rot FungusTrametes hirsuta BYBF

作　　者：周喜燕[1] 谢益民[1,2] 杨海涛[1] 刘冰[1] 陈超[1] 

机构地区：[1]山东轻工业学院制浆造纸省部共建教育部重点实验室,山东济南250353 [2]湖北工业大学化学与环境工程学院,湖北武汉430068

出　　处：《造纸科学与技术》2010年第2期64-67,共4页Paper Science & Technology

基　　金：国家自然科学基金资助项目(20674046)

摘　　要：本实验对白腐菌Trametes hirsuta BYBF产漆酶的发酵条件进行了研究。结果表明,Trametes hirsuta BYBF的最佳培养条件为:培养基初始pH为6.0,培养温度为28℃,碳源为蔗糖,氮源为硝酸铵和酒石酸铵的组合,三角瓶装液量为200/500mL,振荡培养14天左右,酶活达到最大值,为1.37IU/mL。The optimal fermentation conditions for laccase production by white-rot fungus shake-flask cultivation were investigated. The results show that the best growing condition Trametes hirsuta BYBFin of Trametes hirsuta BYBF are as follows ： initial pH of the cuhure medium is 6.0 ; temperature is 28 ℃ ; carbon source is the saccharose and the nitrogen source is the combination of the ammonium nitrate and the ammonium tartrate. The liquid volume was 200mL in 500 mL Erlenmeyer flask. After cultivated with vibration about 14 days, the enzyme activity achieves the maximum, 1.37IU/mL.

关 键 词：白腐菌 TRAMETES hirsuta BYBF 漆酶 酶活 

分 类 号：TS727[轻工技术与工程—制浆造纸工程]