Aspergillus oryzae HN3.042孢子增殖培养基的统计优化(英文)  

Medium Optimization for Conidia Proliferation of Aspergillus oryzae HN3.042 by Statistical Design

作　　者：徐德峰[1] 赵海锋[1] 赵谋明[1] 杨兰[2] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]广东轻工职业技术学院,广东广州510300

出　　处：《现代食品科技》2010年第4期426-432,共7页Modern Food Science and Technology

基　　金：the National Science-Technology Supporting Project for the 11th Five-Year Plan (Nos. 2006BAD27B09-6 and 2007BAK36B03);the Key Technology R&D Program of Guangdong Province (Nos. 2007A010900001 and 2008A010900001)

摘　　要：运用Plackett–Burman和均一设计(UD)对影响Aspergillus oryzae HN3.042孢子增殖的无机盐进行筛选和优化。在筛选的11种无机盐中,KH2PO4,(NH4)2SO4,MgSO4和FeCl3对孢子繁殖有显著正效应。在此基础上,运用UD设计进行添加量优化,孢子增殖最大程度时的无机盐组成为:KH2PO4,4.57g/kg;(NH4)2SO4,1.96g/kg;MgSO4,1.72g/kg和FeCl3,0.64g/kg。在此条件下,孢子数达到1.07×1011个/g干曲,较未优化前提高了66.13%;同时,曲中的中性蛋白酶活力也提高了14.39%。A statistical methodology, combining Plackett-Burman design （PBD） with uniform design （UD）, was applied to screen and optimize the concentrations of different inorganic salt components in solid seed culture medium for conidia proliferation of Aspergillus oryzae HN3.042. Among the eleven inorganic salts investigated with PBD, KH2PO4, （NH4）2SO4, MgSO4 and FeCl3 showed significant positive effects on conidia proliferation. UD was used for optimization of those selected inorganic salts to stimulate the conidial proliferation. By step-wise analysis to the data from UD, a second-order polynomial model was established to identify the relationship between the conidia number and inorganic salts screened. The optimum combination of inorganic salts for conidial proliferation was obtained with the nonlinear optimization algorithm as follows： KH2PO4, 4.57 g/kg; （NH4）2SO4, 1.96 g/kg; MgSO4, 1.72 g/kg and FeCl3, 0.64 g/kg. A maximum conidia number of 10.73 billion conidia/g dry seed koji was demonstrated by confirmatory experiment, which was 66.13% higher than that of the number growing in basal medium. In addition to the promotion of conidia proliferation in seed koji culture stage, the optimum combination of inorganic salts simultaneously increased the neutral protease activity by 14.39% in resulting koji culture stage.

关 键 词：ASPERGILLUS ORYZAE HN3.042 孢子增殖 无机盐 Plackett–Burman设计 均一设计 

分 类 号：Q93-3[生物学—微生物学]