CYR61/CCN1调控良性前列腺增生的血管新生  

Regulation of CCN1 on angiogenesis in benign prostatic hyperplasia

作　　者：张祥华[1] 郭超铭[1] 刘达琪[1] 

机构地区：[1]北京大学第一医院泌尿外科北京大学泌尿外科研究所,100034

出　　处：《中华临床医师杂志（电子版）》2010年第4期19-22,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(30672098)

摘　　要：目的为了探讨与良性前列腺增生(BPH)临床进展相关的分子病理学机制,研究观察了富半胱氨酸61(CYR61)/CCN1在症状性和无症状性BPH组织中的表达和定位,以及与微血管密度(MVD)的相关性。方法无症状性BPH标本10例与症状性BPH标本38例用于本研究。采用免疫组织化学法检测CCN1和CD31蛋白的表达。利用原位杂交技术检测CCN1mRNA的表达。利用免疫荧光双染法检测CCN1和CD31的同步表达。结果在无症状性和症状性BPH标本中,CCN1蛋白以及mRNA表达分别为68.4%与60%(P>0.05),47.4%与40%(P>0.05)。MVD评分在无症状性和症状性BPH标本中分别是19.2和27.6(P<0.01)。在症状性BPH中,MVD评分在不同前列腺体积中有所不同(P<0.01),同时CCN1表达也同(P<0.05)。免疫荧光双染观察证实了BPH中CCN1和CD31的同步表达。结论以上结果提示MVD所反映的血管新生和BPH的进展可能有一定关系,CCN1对症状性BPH中出现的血管新生具有调控作用。Objective The purpose of this study is to investigate possible role of CCN1 and angiogenesis in the pathogenesis and progression of benign prostatic hyperplasia（BPH）. Methods The study included 10 asymptomatic and 38 symptomatic BPH samples. Expression of CCN1 protein and mRNA was examined by immunostaining and in situ hybridization,respectively. The situations of angiogenesis were evaluated by microvessel density（MVD）through CD31 immunostaining. Co-localization of CCN1/CYR61 with CD31 protein was observed by double label immunofluorencesence. Results Expression of CCN1 protein was 68.4% in symptomatic and 60% in asymptomatic BPH samples（P〉0.05）. Expression of CCN1 mRNA was 47.4% in symptomatic and 40% in asymptomatic BPH samples（P〉0.05）. The value of MVD was 27.6 in symptomatic BPH and 19.2 in asymptomatic BPH samples（P〈0.01）. In symptomatic BPH,MVD was closely related to the prostate volume（P〈0.01）,but not related to patient age（P〉0.05）. In symptomatic BPH,CCN1 protein expression was closely related to MVD and the prostate volume（P〈0.05）. Co-localization of CCN1 and CD31 was noted in both asymptomatic and symptomatic BPH samples,which had shown CCN1 or CD31 expression,respectively. Conclusions It is suggested that angiogenesis as reflected by MVD may be closely related to the progression of BPH,and CCN1 may improve angiogenesis in the process of BPH development.

关 键 词：前列腺增生 新生血管化 病理性 胞间信号肽类和蛋白质类 微血管密度 

分 类 号：R697.3[医药卫生—泌尿科学]