CVTree在454高通量测序分析菌群结构中的应用  

The application of CVTree in structural analysis of microbial communities by 454 pyrosequencing

作　　者：华蔚颖[1] 徐昭[2] 张梦晖[1] 李旻[1] 张晨虹[1] 赵立平[1] 

机构地区：[1]上海交通大学生命科学技术学院微生物分子生态与生态基因组学实验室,上海200240 [2]复旦大学理论生命研究中心,上海200433

出　　处：《中国微生态学杂志》2010年第4期312-316,共5页Chinese Journal of Microecology

基　　金：863专题项目(2008AA02Z315);自然基金项目(30730005;20875061);上海市科委国际合作项目(075407001)

摘　　要：目的探究将基于短串频度的CVTree方法用于反映菌群结构的16S rRNA基因的454高通量测序数据分析的可行性,为快速分析高通量菌群结构数据提供新的方法。方法对一个四世同堂的中国家庭7名成员肠道菌群和不同基因型及饮食类型的小鼠肠道菌群用454高通量方法获得16S rRNA基因的V3区的测序数据,用CVTree的方法进行菌群结构的比较分析。结果通过选取合适的短串长度,CVTree的方法能准确检测到各样本间的聚类关系,其结果与之前文献报道的基于Unifrac算法的结果相一致。结论CVTree能快速、有效地处理16S rRNA基因的454高通量测序数据,实现对不同菌群结构相似性的比较分析。Objective To explore the feasibility of CVTree application in structural analysis of microbial communities by 454 pyrosequencing.Method The CVTree was applied to two datasets to perform structural comparison of microbial communities：（1） 454 pyrosequencing data of gut microbiomes of a four-generation,seven-member Chinese family; （2） 454 pyrosequencing data of gut microbiomes of mice with different genotypes and diets.Result When suitable K-tuple length was selected,the CVTree reflected the relationship among the samples which were in good agreement with those reported in the previous studies based on Unifrac analysis. Conclusion Our study shows the ability of CVTree in structural analysis of microbial communities based on 454 pyrosequencing with high efficiency and effectiveness.

关 键 词：CVTree 454高通量测序 菌群结构 

分 类 号：Q-31[生物学]