安徽省猪圆环病毒2型感染的流行病学调查  

Epidemiologic investigation on porcine circovirus type 2 in Anhui

作　　者：李超[1] 魏建忠[1] 淩英济 谢倩[3] 孙裴[1] 李郁[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]安徽巢湖畜牧兽医局,巢湖238000 [3]安徽省产品质量监督检验研究院,合肥230051

出　　处：《中国微生态学杂志》2010年第4期330-332,336,共4页Chinese Journal of Microecology

基　　金：国家科技支撑计划项目(2008BADC1B02);安徽省"十一五"科技攻关项目(08010302155)

摘　　要：目的了解安徽地区猪群中猪圆环病毒2型(PCV2)的感染状况。方法应用酶联免疫吸附试验(ELISA)对采自安徽省14个地(市)的468份血清样品进行PCV2抗体检测,并运用PCR技术同步进行PCV2核酸检测。结果PCV2抗体检测的平均阳性率为74.36%,且阳性率随猪的日龄增长而升高,皖南地区的阳性检出率明显高于淮北和江淮两个地区,PCR与ELISA检测结果的符合率为99.37%。结论安徽省猪群中普遍存在PCV2的感染,病毒血症与抗体共存现象显著。Objective To understand the infection of porcine circovirus type 2 （PCV2） in Anhui province. Method 468 serum samples from 14 areas in Anhui were detected for antibodies against PCV2 by ELISA and for PCV2 by PCR.Result Average positive ratio of antibodies against PCV2 in 468 serum samples were 74.36%. The positive ratio of antibodies against PCV2 increased as pig grew in days. The positive ratio of antibodies against PCV2 in southern Anhui was significantly higher than that in areas north of the Huai River and in areas between Yangtze River and Huai River. There are 99.37% conformity between PCR and ELISA. Conclusion The infection of PCV2 exist widespreadly in pigs in Anhui. There is the phenomenon of co-existence between viremia and antibody against PCV2.

关 键 词：猪圆环病毒2型 流行病学 ELISA PCR 

分 类 号：S852.65[农业科学—基础兽医学]