基于“方证相关”理论的治“痹”经方调控TLR/TRAF信号通路的比较研究  

Comparative study of regulation of TLR/TRAF signaling pathway of classic priscriptions that deals with arthralgia syndrome based on relevent theory of priscription and syndrome

作　　者：徐世军[1,2,3,4] 李磊[3,4] 张文生[3,4] 王永炎[5] 

机构地区：[1]成都中医药大学药学院,四川成都610075 [2]教育部中药材标准化重点实验室,四川成都611137 [3]中药资源保护与利用北京市重点实验室,北京100875 [4]北京师范大学资源学院中药资源与资源药物研究所,北京100875 [5]中国中医科学院,北京100700

出　　处：《中国中药杂志》2010年第8期1025-1029,共5页China Journal of Chinese Materia Medica

基　　金：国家中医药管理局中医科技进步工程专项(06-07ZQ12)

摘　　要：目的:在方证相关理论指导下,比较乌头汤、桂枝芍药知母汤和白虎加桂枝汤调控佐剂性关节炎大鼠脾脏TLR2,TRAF6,Faslg基因表达的差异。方法:建立佐剂性关节炎大鼠模型,采用实时荧光定量PCR技术,以18 sRNA为内参基因,用SYBR G reen I染料法检测脾脏TLR2,TRAF6,Faslg基因相对表达,2-ΔΔCT法进行进行计算和数据分析。结果:佐剂性关节炎大鼠脾脏TLR2,TRAF6,Faslg基因均较空白组显著高表达,乌头汤、桂枝芍药知母汤和白虎加桂枝汤各剂量均能显著抑制或降低模型TLR2,TRAF6,Faslg基因的异常高表达,临床等效剂量时降低的TLR2和Faslg基因表达作用由强到弱依次是乌头汤>桂枝芍药知母汤>白虎加桂枝汤,但降低TRAF6作用强度趋势刚好与TLR2和Faslg基因相反。结论:乌头汤、桂枝芍药知母汤和白虎加桂枝汤均对佐剂性关节炎大鼠脾脏异常高表达TLR2,TRAF6,Faslg基因有下调作用,但强度存在差异,与药效学和调节T细胞亚群趋势较为一致,提示对TLR2/TRAF6信号通路和凋亡受体Faslg基因下调可能是3个经方药效学及对外周T细胞亚群的不同调节强度的原因之一。Objective： Based on relevent theory of priscription and syndrome,to compare the gene expression differences of TLR2,TRAF6,and Faslg with adjuvant arthritis in rat spleen among Wutou decoction,Guizhi Shaoyao Zhimu decoction and Baihu Guizhi decoction.Method： The experiment animal model of adjuvant arthritis in rats was established.Relative expression amount of TLR2,TRAF6,and Faslg in rats spleen was detected by SYBR Green I dye methods and implementation of fluorescence quantitative PCR technology with 18sRNA as an internal gene.2-ΔΔCT method was used for computing and data analysis.Result： TLR2,TRAF6,and Faslg gene in adjuvant arthritis rat spleen was significantly higher than those in the blank group.The various doses of Wutou decoction,Guizhi Shaoyao Zhimu decoction and Baihu Guizhi decoction can significantly inhibit or reduce the abnormally high expression of TLR2,TRAF6,and Faslg genes.The gene expression level caused by three decoctions mentioned above was followed by strong to weak as Wutou decoction,Guizhi Shaoyao Zhimu decoction and Baihu Guizhi decoction with the clinical equivalent dose,but the strength of the trend to reduce the role of TRAF6 is just the opposite with the TLR2 and Faslg genes.Conclusion： Wutou decoction,Guizhi Shaoyao Zhimu decoction and Baihu Guizhi decoction can reduce the abnormally high expression of TLR2,TRAF6 and Faslg in rat spleen with adjuvant arthritis,but the differences of intensity exist and remain relatively consistent with that of pharmacodynamics and regulation trends of T cell subsets.Results suggest that the suppression of TLR2/TRAF6 signal pathway and apoptosis Faslg receptor gene may be the reasons that the pharmacodynamics of three decoctions on peripheral T cell subsets in regulating intensity was different.

关 键 词：乌头汤 桂枝芍药知母汤 白虎加桂枝汤 TLR/TRAF信号通路 Faslg 

分 类 号：R255.6[医药卫生—中医内科学]