参莲提取物对TNF-α刺激下单核细胞株THP-1功能的影响  

Extracts of Danshen and Chuanxinlian on human monocytic line THP-1 induced by TNF-α

作　　者：李玉洁[1] 杨庆[1] 翁小刚[1] 朱晓新[1] 王怡薇[1] 刘小霓[1] 韩晓[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2010年第8期1030-1033,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(30973901);国家直属科研院所技术开发研究专项资金项目(NCSTE-2007-JKZX-301);中国中医科学院自主选题项目(ZZ2006117)

摘　　要：目的：观察参莲（SL）提取物对炎症因子刺激下单核细胞功能的影响。方法：SL提取物预孵育,以TNF-α20μg.L-1为刺激因子,观察SL提取物对单核细胞株THP-1黏附、迁移、摄脂、分泌功能变化的影响。结果：SL1-3（0.5-2 g.L-1）可明显降低HUVEC和THP-1的黏附率,与模型组比较有显著性差异。SL1-5（0.125-2 g.L-1）均可显著减少THP-1迁移细胞数,与模型组比较有显著性差异。SL1-5（0.125-2 g.L-1）均可显著降低细胞内TC值。SL 1（2 g.L-1）可显著降低细胞上清中异常增高的IL-6值,同时升高IL-10的含量,其他剂量未见明显作用。结论：SL提取物对TNF-α刺激下单核细胞黏附、迁移、摄脂、分泌功能均有显著影响,其作用可能是SL提取物干预AS早期炎症反应的机制之一。Objective： To investigate whether extracts of Danshen and Chuanxinlian（SL） could promote the function recovery in pre-monocytic cell line THP-1 induced by TNF-α.Method： SL extracts（0.125-2 g·L-1） were used to incubate THP-1 for 24 h before stimulation with TNF-α（20 μg·L-1）,the adhesion,migration,lipid uptake and secretion of THP-1 were observed.Result： SL（0.5-2 g·L-1） had obvious effect on decreasing the THP-1 adhesion.The number of passed membrane was much fewer than that of control cells in SL（0.125-2 g·L-1）.SL（0.125-2 g·L-1） reduced the total cholesterol content significantly.The levels of IL-6 in SL（2 g·L-1） were significantly decreased,and IL-10 was increased than that before the treatment.Conclusion： SL extracts could promote the function recovery such as adhesion,migration,lipid uptake and secretion of THP-1 induced by TNF-α,which probably is one of the mechanisms of inhibit the inflammatory reaction in initiation and development of AS.

关 键 词：动脉粥样硬化 参莲提取物 炎症 THP-1 

分 类 号：R285.5[医药卫生—中药学]