连翘及其主要有效成分槲皮素体外抗人巨细胞病毒的实验研究  

An in vitro study of anti-human cytomegalovirus effect of Forsythia suspensa and its main active ingredient quercetin

作　　者：张丹丹[1] 方建国[1] 陈娟娟[1] 万进[1] 张亚伟[1] 尹宗智[1] 王楠[1] 陈素华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,湖北武汉430030

出　　处：《中国中药杂志》2010年第8期1055-1059,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30672243)

摘　　要：目的:研究连翘及其主要有效成分槲皮素体外抗人巨细胞病毒作用和细胞毒性。方法:用细胞病变法和MTT法,检测连翘、槲皮素抗人巨细胞病毒的最大无毒浓度、最小有效浓度和治疗指数,并与更昔洛韦进行比较。结果:更昔洛韦、连翘及槲皮素的最大无毒浓度分别为10,30,30 mg.L-1,最小有效浓度分别为10,30,0.3 mg.L-1,治疗指数分别为1,1,100。结论:槲皮素抗HCMV效果大大高于更昔洛韦和连翘,细胞毒性与连翘相同、比更昔洛韦低。连翘尤其是槲皮素具有良好的体外抗人巨细胞病毒(HCMV)效果,有望成为临床抗HCMV的药物,值得进一步深入研究。Objective： To study the in vitro anti-human cytomegalovirus effect and the cytotoxicity of Forsythia suspensa and its main active ingredient quercetin.Method： The 0% toxic dose（TD0）,minimum effective concentration（MEC） and therapeutic index（TI） of anti-human cytomegalovirus activity by F.suspensa and quercetin were detected with the cytopathic assay and MTT method.Ganciclovir was used as the control drug for comparison.Result： The TD0 of ganciclovir,F.suspensa and quercetin were 10,30,30 mg·L-1,the MEC were 10,30,0.3 mg·L-1,TI were 1,1 and 100,respectively.Conclusion： The anti-human cytomegalovirus effect of quercetin is much higher than ganciclovir and F.suspensa,and the cytotoxicity is equivalent to F.suspensa but lower than ganciclovir.Therefore,quercetin has the potential advantages of anti-human cytomegalovirus effect.

关 键 词：连翘 槲皮素 人巨细胞病毒 

分 类 号：R285[医药卫生—中药学]