Visfatin在油酸诱导的SW872脂肪细胞胰岛素抵抗中的作用  

Roles of Visfatin on Insulin Resistance in SW872 Adipocytes Induced by Oleic Acid

作　　者：李瑞珍[1] 马新瑜[1] 卢慧玲[2] 王笑犁[2] 温宇[2] 胡秀芬[2] 林汉华[2] 王宏伟[2] 

机构地区：[1]武汉市儿童医院内分泌科,武汉430016 [2]华中科技大学同济医学院附属同济医院儿科,武汉430030

出　　处：《实用儿科临床杂志》2010年第7期476-478,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(30600213)

摘　　要：目的探讨Visfatin在油酸诱导的SW872成熟脂肪细胞胰岛素抵抗(IR)中的作用。方法体外培养SW872前脂肪细胞,当细胞完全汇合后,加入0.6mmol·L-1油酸诱导分化48h,此时SW872前脂肪细胞被诱导分化为成熟脂肪细胞,以SW872成熟脂肪细胞为研究对象,采用1.0mmol·L-1油酸诱导SW872成熟脂肪细胞产生IR。将其分为正常对照组(成熟SW872脂肪细胞)和IR组(采用1.0mmol·L-1油酸诱导SW872成熟脂肪细胞产生IR)。采用2-脱氧-[3H]-右旋葡萄糖掺入法检测2组SW872成熟脂肪细胞基础状态(基础状态组)、Visfatin和Insulin刺激状态(Visfatin和Insulin刺激组)下的葡萄糖转运能力。结果1.在正常对照组中,Visfatin和Insulin刺激组SW872成熟脂肪细胞葡萄糖转运率显著增加,分别是基础状态组的1.10倍(P<0.01)和1.34倍(P<0.01)。2.在IR组中,SW872成熟脂肪细胞的葡萄糖转运率均显著下降。与正常对照组比较,Visfatin刺激组和基础状态组的葡萄糖转运率分别减少12.30%(P<0.01)和9.73%(P<0.05);Insulin刺激组和基础状态组的葡萄糖转运率分别减少26.81%(P<0.01)和10.50%(P<0.05)。3.IR组内比较,Insulin刺激组与基础状态组比较葡萄糖转运率差异无统计学意义(P>0.05);Visfatin刺激组较基础状态组的葡萄糖转运率增加了6.9%(P<0.05)。结论Visfatin可促进SW872成熟脂肪细胞的葡萄糖转运,增加胰岛素敏感性,改善IR。Objective To explore the roles of Visfatin on insulin resistance（IR） in cultured SW872 matured adipocytes induced by oleic acid.Methods SW872 preadipocytes were cultured in vitro and induced to differentiated by 0.6 mmol·L-1 oleic acid for 48 h.Then the SW872 preadipocytes were differentiated into the matured adipocytes.There were 2 group in the study,normal control group：normal SW872 matured adipocytes;IR group：the normal SW872 matured adipocytes were induced by 1.0 mmol·L-1 oleic acid for 24 h.Glucose transport was assessed by[3H]-2-deoxy glucose uptake.Results 1.Glucose transport stimulated by Visfatin and Insulin in normal control group were significantly increased by 110% （P0.01） and 134% （P0.01） compared with basis state group,respectively.2.In IR group,basic glucose transport was reduced by 9.73% （P0.05）,glucose transport stimulated by Visfatin was reduced by 12.30 % （P0.01） compared with normal control group.Glucose transport stimulated by Insulin was reduced by 26.81 % （P0.01）,basic glucose transport was reduced by 10.50% （P0.05） compared with normal control group.3.In IR group,glucose transport stimulated by Insulin was not increased significantly （P0.05） compared with the same basis state group;Glucose transport stimulated by Visfatin was significantly increased by 6.9%（P0.05） compared with the same basis state group.Conclusion Visfatin can promote glucose transport and improve IR in SW872 matured adipocytes.

关 键 词：VISFATIN 脂肪细胞 胰岛素 油酸 葡萄糖转运 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]