核转录因子κB在5/6肾切除大鼠肾组织中的表达规律  

The expression pattern of NF-kB in the remnant kidney of 5/6 subtotal nephrectomized rats

作　　者：张周沧[1] 苏震[1] 刘娟[1] 

机构地区：[1]温州医学院附属第一医院肾内科,325000

出　　处：《浙江医学》2010年第2期176-179,共4页Zhejiang Medical Journal

基　　金：浙江省自然科学基金资助项目（Y205651）;浙江省留学人员科技活动择优资助项目（温人外[2007]2号文件）

摘　　要：目的 探讨5/6肾切除大鼠模型肾组织中核转录因子κB（NF-κB）的表达规律.方法 将48只雄性SD大鼠随机分为5/6肾切除模型组（24只）和假手术组（24只）,两组分别于术后第2周、第6周、第12周和第16周时测定血肌酐（Scr）,采用凝胶电泳迁移率实验检测肾组织NF-κB活性的表达情况,同时于光镜下观察肾脏组织形态学改变,并计算肾小球硬化指数（GSI）.结果 模型组各时点的Scr水平均较相同时点的假手术组显著增高（均P〈0.01）;模型组大鼠随着时间点延长Scr水平逐渐升高,以术后第16周时最高（均P〈0.05）.模型组术后第12周时可见肾小球体积增大、中重度系膜增生、部分毛细血管腔狭窄,偶见肾小球节段硬化;术后第16周时可见多数肾小球节段硬化,少数肾小球球性硬化;模型组术后第16周时的GSI较第12周时增高（P〈0.05）.模型组各时点的NF-κB水平均较相同时点的假手术组显著增高（均P〈0.01）;模型组的NF-κB水平在术后第2周、第6周、第12周、第16周时的信号呈递增趋势,以术后第16周时表达水平最高（均P〈0.05）.残肾组织NF-κB的活性表达与Scr和GSI呈正相关.结论 在5/6肾切除大鼠模型术后第2周至第16周中,肾组织NF-κB的活性随着肾功能减退而逐渐增强.Objective To examine the expression pattern of NF-kB in the remnant kidney of 5/6 subtotal nephrotomized rats. Methods Forty male Sprague-Dawley rats were randomly divided into 2 groups： 24 rats received 5/6 subtotal nephrectomy （5/6 STNx group） and other 24 received sham operation （sham group）. At 2, 6, 12 and 16 weeks after the 5/6 subtotal nephrectomy, 6 rats from 5/6 STNx and sham groups were sacrificed respectively. The expression of NF-KB in renal tissue was measured by electrophoretic mobility shift assay （EMSA）, and serum creatinine （Scr） levels were also measured. The pathology of kidney was observed with light microscope and the glomerulosclerosis index （GSI） was calculated. Results Compared with the sham group the Scr levels in 5/6 STNx group gradually increased at all time points. In the 5/6 STNx group glomerulosclerosis was observed at the 12 week and markedly at the 16 week after subtotal nephrectomy. Compared with that in the sham group the expression of NF-kB in renal tissue gradually increased in 5/6 STNx group. The expression of NF-kB was correlated with Scr levels and GSI. Conclusion The expression of renal NF-kB gradually increases following renal function deteriorates in 5/6 subtotal nephrectomized rats.

关 键 词：5/6肾切除 核转录因子ΚB 凝胶电泳迁移率实验 

分 类 号：R277.525[医药卫生—中医学]