江门株诺如病毒重组鉴定及分析  

Identification and analysis of recombinant norovirus strain of Jiangmen

作　　者：宋灿磊[1] 戴迎春[1] 胡贵方[1] 向文龙[1] 聂军[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院流行病学系,广州市510515

出　　处：《实用医学杂志》2010年第7期1104-1106,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助课题(编号:30700716;30901992);南方医科大学公共卫生与热带医学院院长基金(编号:GW200801-039)

摘　　要：目的:对诺如病毒(norovirus,NoV)NoV/Jiangmen056/2006/china和NoV/Jiangmen380/2006/china株进行重组鉴定分析。方法:用JV12Y/JV13I、COG2F/GⅡSKR、JV12Y/GⅡSKR三对引物对NoV基因组不同区域分别进行RT-PCR,PCR产物纯化回收、测序,利用ClustalX、MEGA4.1、SimPlot3.5.1软件分析其基因序列。结果:NoV/Jiangmen056/2006/china株由Lordsdal株的RdRp区和Mexico株的capsid区重组而成,而NoV/Jiangmen380/2006/china株由NLV/VannesL169/2000株的RdRp区和SaKaeo-53株的capsid区重组而成。结论:NoV/Jiangmen056/2006/china和NoV/Jiangmen380/2006/china是两种不同型别NoV重组株,而后者是国内首次检出的新型GⅡ.bNoV重组株,上述结果丰富了我国重组NoV的研究资料。Objective To identify and analyze the recombinant norovirus （NoV）/Jiangmen056/2006/China and NoV/Jiangmen380/2006/China. Methods Different regions of NoV genome were respectively amplified by primers of JV12Y/JV13I, COG2F/G II SKR and JV12Y/G II SKR using RT-PCR. The products were purified, sequenced and then analyzed by softwares of Clustal X, MEGA4.1, and SimPlot3.5.1. Results The NoV/Jiangmen056/2006/China strain was identified as a recombinant of RNA-dependent RNA polymerase （RdRp） region of Lordsdal strain and capsid region of Mexico strain, and NoV/Jiangmen380/2006/China strain was a recombinant of RdRp region of NLV/ VannesL169/2000 strain and capsid region of SaKaeo-53 strain. Conclusion NoV/Jiangmen 056/2006/China strain and NoV/Jiangmen380/2006/China strain are two different NoV recombinant strain, and NoV/Jiangmen380/2006/ china strain is a novel G II .b NoV recombinant strain, which may supply important information to study the NoV recombination in China

关 键 词：诺罗病毒 重组 RNA依赖RNA聚合酶 衣壳 开放阅读框架 

分 类 号：R450[医药卫生—治疗学]