冠心病患者外周血T淋巴细胞上PD-1的表达及意义  

Expression of PD-1 on peripheral blood T lymphocyes in patients with coronary heart disease

作　　者：赵欢[1] 徐琳[2] 魏向龙[1] 缪绯[1] 刘芃[1] 张紫微[1] 刘映峰[1] 

机构地区：[1]南方医科大学珠江医院心内科,广州市510282 [2]广州军区广州总医院心内科,510010

出　　处：《实用医学杂志》2010年第7期1143-1146,共4页The Journal of Practical Medicine

基　　金：广东省自然科学基金(编号:815105101000062;2157000068);中国博士后科学基金(编号:20080440215)

摘　　要：目的:研究程序性死亡因子-1(PD-1)在冠心病患者外周血T淋巴细胞上的表达,初步探讨其在动脉粥样硬化发生发展中的意义。方法:选择冠心病患者(55例)为研究对象,其中稳定性心绞痛患者(SA组)27例,急性冠脉综合征患者(ACS组)28例。30例经冠脉造影证实的非冠心病患者为正常对照组。分别用免疫荧光、流式细胞术及RT-PCR检测PD-1蛋白及mRNA在T淋巴细胞上的表达情况。结果:与正常对照组相比,ACS组、SA组外周血T淋巴细胞上PD-1蛋白及mRNA的表达均明显降低(P<0.01),ACS组与SA组比较差异无统计学意义。结论:PD-1在冠心病患者T淋巴细胞上的表达明显降低,这可能在冠状动脉粥样硬化的发生及发展中起到作用。Objective To investigate the expression of programmed death-1 （PD-1） on peripheral blood T cells （PBTCs） in patients with coronary heart disease and the role of this expression in genesis and development of atherosclerosis. Methods 27 patients with stable angina （SA）, 28 with acute coronary syndrome （ACS）, and 30 control subjects with angiographically confirmed noncoronary heart disease were included in this study. The expressions of PD-1 mRNA and protein in PBTCs were detected by direct immunofluorescence, flow cytometry, and half-quantitative RT- PCR, respectively. Results The expression level of PD- 1 was markedly lower in the group with SA or ACS than in the control group （P 〈 0.01 for both comparisons）, but did not differ significantly between SA and ACS （P 〉 0.05）. Conclusions The expression of PD-1 on peripheral blood T cells in patients with coronary heart disease is significantly down-regulated, which may play a role in the pathogenesis and development of atherosclerosis.

关 键 词：冠心病 PD-1 动脉粥样硬化 

分 类 号：R541.4[医药卫生—心血管疾病]