单重和多重脑震荡鼠八臂迷宫参考记忆与工作记忆动态变化研究  

A study on reference memory and working memory of pure cerebral concussion and multiple cerebral concussion by 8-arm radial maze in rats

作　　者：曹珍珍[1] 于建云[1,2] 朱乔[1] 郭泽云[1] 杨力[3] 张桓[1] 曲延玉[1] 

机构地区：[1]昆明医学院法医学院,昆明650031 [2]昆明医学院法医学院脑损伤研究室,昆明650031 [3]昆明医学院法医学院组织胚胎教研室,昆明650031

出　　处：《中华行为医学与脑科学杂志》2010年第4期296-299,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：国家自然科学基金（30560041）;云南省自然科学基金资助项目（2005c0026Q,2008CD052）

摘　　要：目的探讨单重和多重脑震荡后24d内学习记忆能力的动态改变。方法成年SD大鼠30只，应用单摆式闭合性脑损伤机械打击装置，复制不同损伤程度的脑震荡大鼠，即单重脑震荡（PCC）和多重脑震荡（MCC）大鼠（n=8），应用八臂迷宫试验观测评价大鼠伤后学习记忆能力。结果与正常组比，（1）伤后摄取食物量下降，PCC组1～11d[（0．00±0．00）个～（2．62±1．76）个]、MCC组1～24d[（0．00±0．00）个～（0．75±1．48）个]均有统计学意义（t≥3．75，P〈0．05）；（2）伤后精神运动抑制即入臂总次数下降，PCC组1～7d、13d[7d（4．87±1．24）次；13d（10．00±2．39）次]、MCC组1～8d、22d[8d（4．25±5．03）次，22d（9．37±4．20）次]有统计学意义（t≥3．75，P〈0．05）；（3）伤后参考记忆即人有食物臂次数先降后恢复，PCC组1～7d[（0．50±0．75）次，（3．00±1．06）次]等、MCC组1～19df（1．88±2．10）次，（2．50±2．44）次]有统计学意义（t≥3．50，P〈0．05）；（4）工作记忆即重复人无食物臂的次数出现延迟性损伤，PCC组1～6d、10～23d[（0．00±0．00）次，（4．25±3．05）次]、MCC组1～4d、6d、9～13d、15～16d、19～22d[（0．25±0．46）次，（3．12±2．87）次]有统计学意义（t≥3．52，P〈0．05）。结论单重和多重脑震荡鼠在八臂迷宫实验中，摄食功能、精神运动、参考记忆与工作记忆在伤后不同时间表现出严重受损，且前三者的损伤程度与脑震荡损伤次数呈正相关。Objective To observe learning and memory behavior changes of one time cerebral concussion called pure cerebral concussion （PCC） and three times cerebral concussion called multiple cerebral concussion （MCC） after injured 24 days in rats. Methods A metallic pendulum striker device was deployed to duplicate PCC and MCC model in SD rats which were the complete closed head injury model. The animals were divided into PCC and MCC groups at random. One control group was used,each group has eight animals （n = 8 ）. One 8-arms radial maze was used to assessed each animal＇ s capabilities, that is, spatial reference memory, working memory, spirited activity and take in food. Results Compared with control group, there were some significance （P 〈 0.05 ） in both experiment groups post injury,that was, （1） The food intake decreased,PCC group from the 1st to the 1 lth day （from 0.00 ±0.00 to 2.62 ±1.76） after injury,MCC group from the 1st day to the 24th day（from 0.00 ± 0.00 to 0.75 ± 1.48） after injury. （2） Spirited activity depressed,PCC group on the 1st to the 7th,13rd day（from 4.87 ± 1.24 to 10.0 ± 2.39 ） after injury, MCC group on the 1 st to 8th, 22nd day （ from 4.25 ±5.03 to 9.37 ±4.20） after injury. （3）The spatial reference memory was lower in early then gradually increased, PCC group on the 1 st to 7th day （ from 0.50 ± 0.75 to 3.00 ±1.06） after injury, MCC group from the 1 st to 19th day （ from 1.88 ± 2.10 to 2.50 ± 2.44） after injury. （4）The working memory was delaying damaged,PCC group from the 1st to the 6th day and the 10th to the 23rd day（ from 0.00 ± 0.00 to 4.25 ± 3.05 ） after injury, MCC group on the 1 4th,6th,9 - 13th,15th,16th,19th -22nd day （from 0.25 ±0.46 to 3.12 ±2.87） after injury. Conclusion The injured rats＇capability of spatial reference memory, working memory, spirited activity and food intake were obviously damaged after CC, and the MCC group＇s capability of spatial reference memory,spirited activity and food inta

关 键 词：单重脑震荡 多重脑震荡 参考记忆 工作记忆 大鼠 

分 类 号：R686[医药卫生—骨科学]