补益肝肾中药对更年期抑郁症模型大鼠5-羟色胺递质系统的影响  

Effect of Chinese medicine of replenishing the liver and kidney on the system of 5-HT in perimenopausal depression model rats

作　　者：肖爱娇[1] 王莉[2] 闵建新[1] 

机构地区：[1]江西中医学院生理学教研室,南昌330004 [2]江西中医学院中医基础理论教研室,南昌330004

出　　处：《中华行为医学与脑科学杂志》2010年第4期300-303,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：江西省自然科学基金资助项目（2007GQY0944）

摘　　要：目的制备更年期抑郁症大鼠模型，观察补益肝肾中药对模型大鼠海马5-羟色胺（5-HT）含量及其5-HT合成限速酶-色氨酸羟化酶2（TPH2）表达的影响，探讨补益肝肾中药防治更年期抑郁症的机理。方法采用雌性SD大鼠，在卵巢切除术的基础上，联合孤养和21d慢性不可预见性的温和刺激（CUMS）制备更年期抑郁症大鼠模型。以高效液相色谱-电化学检测法（HPLC—ECD）测定海马5-HT含量、RT—PCR技术检测中缝核TPH2mRNA表达以及荧光免疫组化检测中缝核TPH2蛋白表达。结果中药组海马5-HT含量高于模型组，但差异无显著性[（2543．06±859．59）ng／g组织，（1845．81±233．55）ng／g组织，F=9．617，P〉0．05]。RT—PCR示中药组中缝核TPH2mRNA相对表达量明显高于模型组[（1．282±0．158），（0．985±0．120），F=3．552，P〈0．05]。荧光免疫组化示中药组TPH2阳性细胞数明显多于模型组[（152．74±68．52）个，（74．12±38．01）个，F=7．939，P〈0．01]。结论补益肝肾中药具有防治更年期抑郁症的作用，提高大鼠中缝核TPH2的表达，促进5-HT的合成，使海马5-HT含量升高，可能是补益肝肾中药防治更年期抑郁症的作用途径之一。Objective To observe the effect of Chinese medicine of replenishing the liver and kidney on the concentration of 5-hydroxytryptamine （5-HT） in hippocampns and the expression of tryptophan hydroxylase 2 （TPH2） in median raphe nuclei in perimenopausal depression model rats and to explore its mechanism. Methods The animal model was established by resetting the ovaries of female rats, then giving isolation-housing in combination with 21 days of chronic unexpected mild stress （CUMS）. High performance liquid chromatogram-electrochemical detection（HPLC-ECD） were employed to measure 5-HT in hippocampus, the hemi-quantitative reverse transcription-polymerase chain reaction（RT-PCR） and fluorescence immunohistochemistry were employed to detect the relative expression of TPH2 mRNA and number of TPH2 immunoreactive neurons in median raphe nuclei. Results The concentration of 5-HT in hippocampus was increased in rats of the medicine group compared with that in the model group （ （ 2543.06 ± 859.59 ） ng/g tissue, （ 1845.81 ± 233.55 ） ng/g tissue, F = 9.617, P 〉 0.05 ）. RT-PCR showed that the relative expression of TPH2 mRNA in medicine group was much higher than that in model group （ （ 1. 282 ±0. 158）, （0. 985 ±0. 120）, F = 3. 552, P 〈 0.05 ）. Immunofluorescence showed that the number of TPH2 immunoreactive neurons in raphe nuclei had statistical significance between medicine group and model group （（152.74 ±68.52） ,（74.12 ±38.01）, F=7.939, P〈0.01）. Conclusion Chinese medicine replenishing liver and kidney have effects of improving perimenopausal depression, increasing the expression of TPH2 in median raphe nuclei, which leads to enhance the synthesis of 5-HT in hippocampus might be one of its mechanisms.

关 键 词：更年期抑郁症 5-羟色胺 色氨酸羟化酶2 

分 类 号：R686[医药卫生—骨科学]