肝乐颗粒对肝纤维化大鼠纤维化指标的影响  

Effect of Ganle Granule on Indices of Hepatic Fibrosis in Fibrotic Liver Rats

作　　者：姜辉[1] 吴芙蓉[2] 薛雪[1] 张家富[1] 

机构地区：[1]安徽中医学院第一附属医院,合肥230031 [2]安徽医科大学药学院,合肥230032

出　　处：《中国中医急症》2010年第4期636-637,共2页Journal of Emergency in Traditional Chinese Medicine

基　　金：安徽中医学院临床科研基金项目(No.2008lcky011)

摘　　要：目的观察肝乐颗粒对肝纤维化大鼠纤维化指标的影响。方法将大鼠随机分为6组,除正常组外,其余各组采用CCl4诱导肝纤维化模型。于造模第7周起,给药组分别灌胃给予相应的受试药物,正常组和模型组灌胃生理盐水,疗程6周。实验结束后,放射免疫法测定血清透明质酸(HA)、层粘蛋白(LN)、Ⅲ型前胶原氨基端肽(PⅢNP)、四型胶原(CⅣ)和转化生长因子-β1(TGF-β1)的含量;RT-PCR技术测定肝组织中Ⅰ型前胶原mRNA的表达。结果肝乐颗粒各剂量组能明显降低肝纤维化大鼠血清HA、LN、PⅢNP、CⅣ和TGF-β1的含量,抑制肝纤维化大鼠肝组织Ⅰ型前胶原mRNA的表达。结论肝乐颗粒能有效抑制CCl4诱导的肝纤维化大鼠纤维化指标的表达。Objective： To observe he change of indexes of hepatic fibrosis in rats after treatment with Ganle Granule. Methods： Rat liver fibrosis had been induced by CCl4 twice a week for 12 weeks. Ganle Granule was used daily intragastric administration at 7 week. The levels of HA, LN, PⅢNP, CIV and TGF - β1 in serum were determinated by radioimmunity. Procollagen Ⅰ mRNA expression in liver tissue was detected by RT - PCR technology. Results： Ganle Granule could significantly reduce HA, LN, PⅢNP, CⅣ and TGF - β1 contents. Meanwhile Ganle Granule could effectively prevent the expression of Procollagen Ⅰ mRNA in liver fibrosis rats. Conclusion： Ganle Granule can effectively inhibit the expression of indexes ahout hepatic fibrosis in fibrotic liver rats.

关 键 词：肝乐颗粒 肝纤维化 大鼠 

分 类 号：R285.5[医药卫生—中药学]