辐射损伤小鼠胸腺组织的FTIR光谱变化与辐射剂量的关系  

Study on Estimation of Radiation Dose Using FTIR in Mice Thymus

作　　者：吴文安[1] 王健生[1] 张佳[1] 莫非[1] 王嗣岑[2] 周苏娜[1] 张明鑫[1] 徐怡庄[3] 石景森[1] 吴瑾光[3] 

机构地区：[1]西安交通大学医学院第一附属医院,陕西西安710061 [2]西安交通大学药学院,陕西西安710061 [3]北京大学化学与分子工程学院,北京100871

出　　处：《光谱学与光谱分析》2010年第5期1189-1191,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30500119)资助

摘　　要：应用傅里叶变换红外(FTIR)光谱分析辐射损伤小鼠胸腺组织随辐射剂量增加的生化变化过程,为临床评估患者辐射剂量提供新的研究思路。结果表明,随着辐射剂量的增加,辐射损伤小鼠胸腺组织FTIR光谱的主要吸收峰位变化表现在照射2,3,5Gy时,在1550,1460,1400及1640cm-1处峰位发生移位(P<0.05),峰高差异表现在:(1)与核酸有关的谱带1085和1236cm-1的峰强比呈下降趋势,(2)与蛋白质有关的谱带1640与1550cm-1的峰强比随剂量增加明显下降,(3)与脂类有关的谱带2958,2925,1460及1400cm-1未发生明显的变化。研究结果表明傅里叶变换红外光谱有望成为临床估算受照剂量的新方法。Fourier transform infrared spectroscopy （FTIR） was applied to study the biochemical changes in the radiation damaged mouse thymus which increased with radiation dose and provided a new method for the estimation of the radiation dose of radiation damaged patients. The results demonstrated that with the dose increasing, the peak positions like 1 550, 1 400, 1 400 and 1 640 cm^-1 at the dose of 2, 3 and 5 Gy showed some difference, and there was obvious variance in the intensity： （1） The intensity ratio of 1 085 to 1 236 cm^-1 related to nucleic acid tended to decrease. （2） The intensity ratio of 1 640/1 550 decreased. （3） The intensity at 2 958, 2 925, 1 460 and 1 400 cm^-1 showed no significant difference. The results suggest that it may be possible for FTIR to become an effective method to estimate the radiation dose in clinic.

关 键 词：辐射剂量 胸腺组织 傅里叶变换红外光谱 

分 类 号：O657.3[理学—分析化学]