三种酚类化合物的三维荧光光谱特性研究  

Excitation-Emission Fluorescence Characterization Study of the Three Phenolic Compounds

作　　者：王欢博[1] 张玉钧[1] 肖雪[1] 金丹[1] 赵南京[1] 殷高方[1] 郭立泉[1] 刘文清[1] 

机构地区：[1]中国科学院安徽光学精密机械研究所,中国科学院环境光学与技术重点实验室,安徽合肥230031

出　　处：《光谱学与光谱分析》2010年第5期1271-1274,共4页Spectroscopy and Spectral Analysis

基　　金：国家(863计划)项目(2007AA061502)资助

摘　　要：三维荧光光谱技术通过在不同激发波长下扫描发射光谱获得荧光强度变化信息,由于其灵敏度高,选择性好,被广泛用于环境中污染物的监测。利用该方法研究3种酚类化合物的荧光光谱特性,在激发波长为240～360nm,发射波长为260～500nm范围内,确定了苯酚、间甲酚和麝香草酚的荧光峰位置分别为272/300,274/300和276/304nm。由于3种酚类物质为同系物,结构相似,因此得到的激发光谱和发射光谱在形状上极为相似。工作液浓度在0.02～1.0mg.L-1范围内,3种酚类物质的浓度与荧光强度之间均呈现较好的线性关系,且检出限达到1μg.L-1。实验结果表明,用三维荧光光谱法可对3种酚类化合物进行定性和定量分析。The fluorescence intensity information was collected by scanning its fluorescence spectra at different excitation wavelengths. Based on its high sensitivity and selectivity, excitation-emission fluorescence can be widely used for detection of pollutants in the environment. The characterizations of the three phenolic compounds were investigated by this method, and the fluorescence peaks of phenol, m-cresol and thymol were confirmed at 272/300 nm, 274/300 nm and 276/304 nm when the excitation and emission wavelength were set in the range of 240-360 nm and 260-500 nm respectively. The excitation and emission spectra of the three phenolic compounds are very similar because of their analogical structure. The intensity of the spectrum has a good linear relationship with the concentration when the solution concentration is between 0.02 and 1.0 mg·L^-1, and the limits of detection can reach at 1 μg·L^-1. Results show that the three compounds can be analyzed qualitatively and quantitatively by excitation-emission fluorescence.

关 键 词：苯酚 间甲酚 麝香草酚 三维荧光光谱 荧光特性 

分 类 号：X830.2[环境科学与工程—环境工程]