中国北方汉族人醛糖还原酶基因C（-106）T多态性与2型糖尿病并发冠心病的相关性研究  

Relationship between Aldose Reductase Gene C （- 10 6） T Polymorphisms and Type 2 Diabetes Mellitus with Coronary Heart Disease in Han Nationality from Northern China

作　　者：夏良裕[1] 张麟[1] 金勋[2] 鄢盛恺[2] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院检验科,北京100730 [2]卫生部中日友好医院检验科,北京100029

出　　处：《现代检验医学杂志》2010年第2期11-14,共4页Journal of Modern Laboratory Medicine

基　　金：人力资源和社会保障部2008年年度留学人员科技活动项目择优资助经费（启动类项目）及中日友好医院科研基金资助项目.

摘　　要：目的探讨中国北方地区汉族人醛糖还原酶（AR）基因C（-106）T基因多态性与2型糖尿病并发冠心病的关系。方法用聚合酶链反应限制性片段长度多态性（PCR—RFLP）技术检测了125例对照者、85例2型糖尿病患者（DM组）和78例糖尿病并发冠心病患者（DM＋CHD组）ARC（-106）T多态性基因型和等住基因频率分布，同时检测了糖化血红蛋白（HbAlc），血糖（Glu），血脂水平。结果三组研究对象的C（-106）T多态性基因型和等位基因频率分布差异无统计学意义（P〉0．05）。CC基因型和CT＋TT基因型组间HbAlc，Glu，血脂水平无明显差异，未发现ARC（-106）T与2型糖尿病并发冠心痛有相关性。结论ARc（-106）T多态性与糖尿病并发冠心病无明显关联性，不是中国北方地区汉族人糖尿病并发冠心病发病的独立危险因素。Objective To explore the effects of the interaction between Aldose Reductase （AR） C（-106）T on the presenee of gene polymorphisms and type 2 diabetes mellitus（T2DM） with coronary heart disease （CHD） in Han nationality from Northern China. Methods Genotypes of AR C（-106）T polymorphisms were analyzed by using polymerase chain reactionrestriction fragment length polymorphism （PCR-RFLP） in 125 unrelated healthy individuals, 85 patients diagnosed of T2DM,and 78 patients of T2DM combined CHD. The relationship of gene polymorphisms of AR C（-106）T and levels of HbAlc,serum llpids,and glucose was also analysed. Results Neither the frequencies of genotypes nor frequencies of alleles of AR C（-106）T polymorpbisms were statistically different among DM patients,DM＋CHD patients and controls （P〉 0. 05）. Serum lipid levels ,glucose and HbAlc presented no significant difference between CC and CT＋TT subgroups. Conclusion The C（-106）T polymorpbisms of AR gene showed no significant correlation with T2DM with CHD. It maybe not the major genetic risk factor of T2DM in Han nationality from Northern China.

关 键 词：醛糖还原酶 基因多态性 糖尿病 冠心病 聚合酶链反应限制性片段长度多态性 

分 类 号：R587.2[医药卫生—内分泌] Q786[医药卫生—内科学]