慢性乙型肝炎患者HBV前C区A1896变异和BCP T1762／A1764双变异与临床相关性分析  

Analysis Chronicity Hepatitis B Patients with HBV Pre-C A1896 Mutation and BCP T1762/A1764 Pairs of Mutations and Clinical Correlation

作　　者：刘成忠[1] 王琴[1] 肖传宇[1] 王建伟[1] 谢强[1] 侯文华[1] 

机构地区：[1]湖北省枣阳市第一人民医院检验科,湖北枣阳441200

出　　处：《现代检验医学杂志》2010年第2期81-83,86,共4页Journal of Modern Laboratory Medicine

摘　　要：目的探讨慢性乙型肝炎病毒（HBV）前C区和基本核心启动子（BCP）突变与HBV—DNA载量之间的关系，明确检测突变的意义。方法PCR扩增HBV—DNA前C区序列，进行PCR产物直接测序，测序HBV感染者血清中HBV前C区A1896突变及BCP T1762／A1764双突变例数。结果31例慢性乙型肝炎患者血清HBV—DNA发生nt1896住核苷酸G—A点突变18例；发生nt1762位A—T16例；发生nt1764位G〉A18例；发生nt1762位A—T与1764位G—A双突变16例；1762住A—T1764位G—A双变异和1896位G～A变异的联合变异频率明显高于单个变异。用SPSS16．0软件分析突变与HBV—DNA载量的关系。前C区A1896变异，BCPT1762／A1764变异均与HBV—DNA载量差异无统计学意义。结论HBV—DNA载量仅能反映实时病毒载量，HBV前C区A1896变异和BCPT1762／A1764双变异在各种病毒载量感染者中存在，仅依靠血清学指标及HBV—DNA载量来判断传染性是不够的。检测HBV—DNA前C区A1896变异和BCPT1762／A1764双变异，可辅助判断传染性及制定抗病毒治疗方案。Objective To explore the relationship between hepatitis B virus （HBV） pre-C region/the basic core promoter （BCP） mutation and the copy numbers of HBV-DNA. Identify the significance of mutation detection. Methods Conventional PCR was done and pre-C region of HBV-DNA was amplified,the PCR products was sequenced directly. 31 cases of hepatitis B patients＇ peripheral blood were drawn and A1896 mutation in HBV pre-C region and the BCP T1762/A1764 double mutations both detected by sequencing. SPSS 16.0 software was used to analysis the relationship between HBV-DNA mutation and the numbers of HBV-DNA. Results 31 cases of hepatitis B serum HBV-DNA occurred nt1896 nucleotide G-A point mutation in 18 cases ;nt1762-bit A-T occurred in 16 cases roccurred nt1764-bit G-A in 18 cases ;occurred nt1762-bit A-T and 1764 G-A pairs of mutations in 16 cases; 1762 A-T1764-bit G-A pairs of mutations and variations of the joint 1896 G-A mutation frequency was significantly higher than the individual variation. Using SPSS 16.0 analysis mutation and HBV-DNA load relationship. Pre-C, A1896 mutation,BCP T1762/A1764 variations related to HBV-DNA load was not statistically significant differences. Conclusion The copies of HBV-DNA can reflect the real-time virus number only,pre-C A1896 mutation and BCP T1762/A1764 dual variation could be helpful for determining the infectious status and the development of anti-viral treatment.

关 键 词：乙型肝炎病毒 基本核心启动子 前C基因 突变 DNA序列分析 

分 类 号：R512.62[医药卫生—内科学] Q754[医药卫生—临床医学]