大内皮素-1的定量ELISA法测定与临床应用  

Enzyme-Linked Immunosorbent Assay for Quantitative Determination of Big Endothelin-1 in Plasma and Its Clinical Application

作　　者：汪隆海[1] 夏芳[1] 陈启松[1] 

机构地区：[1]安徽省含山县人民医院,安徽含山238100

出　　处：《现代检验医学杂志》2010年第2期97-99,共3页Journal of Modern Laboratory Medicine

摘　　要：目的建立血浆大内皮素-1（BigET-1）的定量ELISA法测定，并探讨其应用价值。方法用定量ELISA法测定患者和健康者的血浆BigET-1。结果该法检出限为0．023pmol／L，线性为0．05-10．0pmol／L，平均回收率为99．1％，批内CV为3．10%，批间CV为5．23％。39例心绞痛和33例急性心肌梗死及52例原发性高血压患者的血浆BigET-1值分别为2．89±0．80，4．76±0．84和3．80±0．81pmol／L。结论该法灵敏、特异和准确，血浆BigET-1定量测定是诊断心血管疾病较好的实验指标。Objective To establish enzyme-linked immunosorbent assay（ELISA） for the quantitative determination of big endothelin-1 （Big ET-1 in plasma and to investigate its clinical application. Methods Plasma Big ET-1 concentrations of patients and of normal controls were measured by ELISA. Results The limit of detection（LOD） was 0. 023 pmol/L. The linearity was 0.05-10.0 pmol/L. The average rate of recovery was 99.1%. Within-run CV was 3.10% and between-run CV was 5.26%. The plasma Big ET-1 concentrations of 39 patients with angina pectoris were 2.89＋0. 80 pmol/L,of 33 patients with acute myocardial infarction were 4. 76± 0. 84 pmol/L and of 52 patients with primary hypertension were 3.80 ±0. 81 pmol/L. Conclusion This method is sensitive,specific and accurate. Quantitative detection of Big ET-1 is now better laboratory test for diagnosing cardiovascular diseases of patients.

关 键 词：大内皮素-1 酶联免疫吸附测定 心血管疾病 

分 类 号：R446.62[医药卫生—诊断学]