非浓缩尿蛋白电泳在高血压早期肾损伤诊断中的应用  

Non-concentrated Urine Protein Electrophoresis for the Early Diagnosis of Hypertensive Renal Injury

作　　者：张银辉[1] 萧晓友[1] 张允奇[1] 曾冬梅[1] 

机构地区：[1]广东医学院附属深圳福田医院,广东深圳518033

出　　处：《现代检验医学杂志》2010年第2期113-114,共2页Journal of Modern Laboratory Medicine

摘　　要：目的探讨非浓缩尿蛋白电泳在高血压早期肾损伤诊断中的临床应用。方法采用十二烷基硫酸钠-琼脂糖凝胶电泳（SDS-AGE）对33例高血压患者进行尿蛋白分析。结果尿蛋白经SDS-AGE电泳后，根据尿蛋白电泳图谱，可区分为生理性、肾小管性、肾小球性、混合性蛋白尿。对照组电泳后有3例出现单纯淡染的清蛋白条带。患者组中2例未检出，生理性蛋白尿2例，肾小管性蛋白尿20例，肾小球性蛋白尿5例，混合性蛋白尿4例。结论SDS-AGE非浓缩尿蛋白电泳操作简便，无须浓缩尿液，省时，尿蛋白按分子量大小予以分离，结果清晰，便于保存，能正确的反映尿液内各蛋白组分的含量及变化，对高血压早期肾损伤诊断有较高的临床应用价值。Objective To investigate the non-concentrated urine protein electrophoresis in the diagnosis of hypertensive renal injury early in the clinical application. Methods Sodium dodecyl sulfate-agarose electrophoresis （SDS-AGE） of the 33 cases of hypertension in patients with urinary protein analysis. Results Urinary protein by SDS-AGE could make urine protein electrophoresis patterns and could distinguish between living rational,renal tubular and glornerular,mixed proteinuria. The control group after eleetrophoresis in 3 cases of pure light stained albumin bands. The patient group were not detected in 2 cases, 2 cases of physiological proteinuria, 20 cases of renal tubular proteinuria, glomerular proteinuria 5 cases and 4 cases of mixed proteinuria. Conclusion Non-concentrated urine protein electrophoresis,easy to operate. No need to concentrate urine, time-saving and urinary protein to be separated by molecular weight, resulting in clear and easy to save. Correctly reflected in the urine protein content and composition changes in hypertensive renal injury in early diagnosis have a high clinical value.

关 键 词：十二烷基硫酸钠-琼脂糖凝胶电泳 非浓缩尿 高血压 肾损伤 

分 类 号：R544.1[医药卫生—心血管疾病] R446.112[医药卫生—内科学]