呼吸道合胞病毒肺炎患儿特异性免疫应答指标的测定  

Analysis of Index of Specific Immune Response in Children with Respratory Syncytial Virus Pneumenia

作　　者：王飞[1,2] 周新[1] 宋贵波[1] 丁锋[1] 王春芳[1] 

机构地区：[1]武汉大学中南医院基因诊断中心,武汉430071 [2]湖北省中医院检验科,武汉430061

出　　处：《现代检验医学杂志》2010年第2期147-148,共2页Journal of Modern Laboratory Medicine

摘　　要：目的探讨肺炎患儿外周血中免疫球蛋白和T细胞亚群的变化。方法采用流式细胞术对50例呼吸道合胞病毒（RSV）肺炎患儿及40例正常儿童外周血T细胞亚群进行检测，采用免疫比浊法对免疫球蛋白进行检测。结果肺炎组与对照纽的IgG，IgM，IgA组间比较差异均有统计学显著性意义（P〈0．05）。RSV肺炎组与对照组外周血T细胞亚群CD8差异无统计学显著性意义（P〉0．05），CD4，CD4／CD8比值RSV肺炎组高于对照组，差异有统计学显著性意义（P〈0．05）。结论RSV肺炎患儿存在比较明显的体波免疫功能和细胞免疫功能紊乱，提示这两者在RSV感染所致的肺炎的发病机制上有一定的临床意义，建议在治疗中可采用免疫干预措施纠正机体存在的免疫失衡，以达到预防RSV肺炎患儿复喘甚至发展成为哮喘疾病。Objective To observe the changes of immunoglobulin and T lymphoeytes subsets in peripheral blood of children with respiratory syncytial virus （RSV） pneumonia. Methods Fresh peripheral blood samples of patients in 50 RSV group and 40 healthy children （control group） were collected and detected T lymphocytes subsets hy the flow cytometry,immunoglobulin by Immunoturbidimetry. Results The content of IgG,IgM,IgA were significantly higher in RSV group than that in control group. There was no significant difference on the percentage of CD8^＋ cell between the two groups （P〉 0. 05）. The percentage of CD8^＋ cell and the ratio of CD4^＋/CD8^＋ in RSV pneumonia group were also higher than those in control group（P〈0. 05）. Conclusion There are very significant humoral immunity and cellular immunity dysfunction in children with RSV pneumenia,which indicate that the two indexes have clinic significance in pathogenesis of RSV pneumenia. So intervention study could be taken in therapy to correct the immunity dysfunction to prevent Asthma.

关 键 词：呼吸道合胞病毒 肺炎 免疫应答 体液免疫 

分 类 号：R373.1[医药卫生—病原生物学] R446.62[医药卫生—基础医学]