基于透射式荧光型光纤生物传感器的结构设计  

Structural design of transmission-based optical fiber fluorescence biosensor

作　　者：王以明[1] 张玉玉[1] 陈文[1] 

机构地区：[1]成都理工大学材料与化学化工学院,成都610059

出　　处：《生命科学仪器》2010年第2期61-64,共4页Life Science Instruments

摘　　要：本文设计的基于透射式荧光型光纤生物传感器的样品反应池,采用光学技术和微流控技术、介电电泳及微/纳机械加工技术等,克服了现有同类仪器相对缺乏且多为断点测量的不足。该设计采用的微流控通道和温控系统保证生化样品在模拟生理条件下进行检测,真实地反映生物组分的特性;介电电泳将探针分子聚集到生物生物传感器的反应活性表面,保证靶标分子能探针分子的杂交,有利于微、痕量组分的富集;镜面的锥形腔体结构为聚光装置,然后将光信号耦合到光导纤维,导入光学型光纤生物传感器的检测器加工处理。该设计能缩短响应时间,提高生物传感器的检测灵敏度。Reactor of transmission-based optical fiber fluorescence biosensor was designed using the optical technique, microfluidic tech- nology, dielectrophoresis and micro/nano machining technology, which overcomes the limitation of broken point measurement of optical fiber biosensors. The designed device satisfied the need as an effective tool in optical fiber sensing system and is better than other similar products. Micro-fluidic channels and temperature control system of this design might ensure to detect chemical and biological samples under simulated physiological conditions, and truly reflect the characteristics of biological components. Probe will be gathered to the reactive surface of biosen- sors to ensure the hybridization of targeted molecular and probe molecules by dielectrophoresis, which was in favor of the enrichment of trace components. The experimental device adopts the cone-shaped cavity by mirror-like reflection as spotlight to couple optical signal to the de- tector. The response time of biosensors might be shorten, and the sensitivity was improved.

关 键 词：光纤生物传感器 微流控技术 介电电泳 微/纳机械加工技术 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]