大蒜提取物二烯丙基三硫化物对人膀胱癌T24细胞增殖的影响  

The Effects of Garlic-Derived Dats on Proliferation of Human Bladder Carcinoma T24 Cells

作　　者：王云彬[1] 谢立平[2] 秦杰[2] 郑祥毅[2] 白宇[2] 杨凯[2] 

机构地区：[1]内蒙古医学院第一附属医院泌尿外科,内蒙古呼和浩特010050 [2]浙江大学医学院附属第一医院泌尿外科,浙江杭州310003

出　　处：《疾病监测与控制》2010年第4期193-195,共3页Journal of Diseases Monitor and Control

基　　金：浙江省中医药普通课题研究计划(项目编号:2009CA057)

摘　　要：目的研究大蒜提取物二烯丙基三硫化物(DATS)于体外对人膀胱癌细胞系T24细胞增殖和细胞周期的影响及其可能机制。方法采用MTT法观察不同浓度的DATS作用不同时间后对T24细胞增殖的影响。同时应用集落形成试验观察不同浓度的DATS对T24细胞集落形成的影响。采用流式细胞仪对经不同浓度DATS作用24h后T24细胞进行细胞周期分析。应用Western blotting方法检测细胞周期相关蛋白Cdc25C的表达,以观察DATS对其的影响。结果DATS对T24细胞的增殖有明显的抑制作用,MTT法显示随药物浓度升高和作用时间延长其抑制作用逐渐增强。DATS抑制T24细胞集落形成,随着浓度增高集落形成率逐步下降。经流式细胞仪检测,不同浓度DATS作用后,G2/M期细胞逐渐增多,表明DATS可引起G2/M期停滞。不同浓度DATS作用后,Cdc25C蛋白表达随药物浓度增加。结论DATS能显著抑制T24细胞增殖和集落形成,其抑制呈时间和剂量依赖性。DATS能显著抑制T24细胞中Cdc25C蛋白的表达,这可能是诱导T24细胞G2/M期停滞的分子机制之一。Objective To investigate the effects of diallyl trisulfide（DATS） on growth inhibition,cell cycle and corresponding molecular mechanism of T24 cells.Methods The cytotoxic effect of DATS on T24 cells was determined with varying concentration of DATS treatment by MTT assay.The antiproliferative effect of DATS in T24 cells was determined by anchorage-dependent colony-forming assay.The cell cycle arrest effect of DATS on T24 cells was determined by flow cytometry.The cell cycle modulating protein Cdc25C was determined by immunoblotting.Results Using the T24 human bladder cancer cell line,we found that DATS treatment resulted in dose-dependent and time-dependent inhibition of cellular proliferation and cell viability.There was a drastic decrease in the ability of the T24 cells to form colonies with increasing doses of DATS.As shown by flow cytometry,DATS treatment of the T24 cells resulted in significant G2/M phase cell cycle arrest.As shown by immunoblot analysis,DATS treatment of the T24 cells resulted in significant dose-dependent downmodulation of the protein expression of Cdc25C.Conclusions Our data suggested DATS treatment resulted in a significant dose-and time-dependent inhibition in the growth and colonogenic survival of T24 Cells.Our study also suggests that DATS causes a decrease in the protein levels of the Cdc25C, thereby causing a G2/M-phase arrest of the cell cycle.

关 键 词：膀胱癌 细胞周期 二烯丙基三硫化物 

分 类 号：R318.16[医药卫生—生物医学工程]