甜樱桃品种SSR指纹图谱的构建  

Establishment of Fingerprint for Sweet Cherry Cultivars Using SSR Markers

作　　者：艾呈祥[1] 刘庆忠[1] 李国田[1] 张力思[1] 

机构地区：[1]山东省果树研究所,山东泰安271000

出　　处：《山东农业科学》2010年第4期8-10,共3页Shandong Agricultural Sciences

基　　金：国家"863"计划资助项目(2006AA100108-4-12-9)

摘　　要：采用SSR标记技术构建甜樱桃品种的分子指纹图谱，为甜樱桃品种鉴定提供依据。以“泰山朝阳”、“泰山红日”和“红南阳”等品种为试材，从38对引物中筛选出5对多态性及稳定性高的SSR引物进行PCR扩增。5对引物扩增出的多态性带数在10—15条，平均为12．6条；引物的多态信息量（PIC）在0．8347—0．9020，平均0．8708。依据甜樱桃SSR带型特征，对每对引物生成的不同带型直接编号，简化甜樱桃SSR带型记录方法，并利用每个品种的带型编号，建立其DNA指纹图谱。表明SSR标记技术可用于鉴定甜樱桃种质。Sweet cherry cultivars ＂ Taishanzhaoyang＂, ＂ Taishanhongri＂,＂ Hongnanyang＂ were used as materials in this study. Five pairs of primers were selected from 38 pairs of pre - selected primers for PCR with higher polymorphism and stability. The number of polymorphic bands varied between 10 and 15 among sweet cherry cultivars with an average of 12.6. The mean of polymorphism information content （PIC） value was 0. 8708, ranging from 0. 8347 to 0.9020. Due to the complex SSR patters in sweet cherry, it was difficult to score each band with number 1 and 0. So we scored each SSR pattern with an alphanumeric code. By arranging all of the pattern codes, the fingerprint for every sweet cherry germplasm was established.

关 键 词：甜樱桃 SSR 指纹图谱 

分 类 号：S662.5[农业科学—果树学] Q503[农业科学—园艺学]