应用改进的免疫荧光组织化学染色法对水稻根尖细胞微管结构的观察  

An Improved Immunofluorescence Staining Method for Observing Microtubule Cytoskeleton in Root Tip of Rice

作　　者：计淑霞[1,2] 盖国琛[2] 戴绍军[1,3] 刘炜[2] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150025 [2]山东省农业科学院高新技术研究中心/农业部黄淮海作物遗传改良与生物技术重点开放实验室,山东济南250100 [3]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《山东农业科学》2010年第4期40-42,55,共4页Shandong Agricultural Sciences

基　　金：国家自然科学基金(编号:30870191)资助

摘　　要：植物细胞微管在细胞形态建成中具有重要作用。它体积小,且结构始终处于动态变化之中,因此观察到清晰的微管形态有一定的困难。本实验以水稻根尖为材料,介绍了一种改进的植物细胞微管免疫荧光组织化学染色方法,用此方法可以观察到清晰、完整的植物微管形态,此法对观察其它植物细胞微管结构也具有指导作用。The microtubule （MT） cytoskeleton plays an important role in plant cell morphogenesis. As the volume of MT is very small, and its morphology is always in dynamic changes, it is very difficult to observe a clear pattern of microtubule. In this paper, an improved immunofluorescence staining method was introduced using the root tip of rice as experimental materials, and the results showed that a clearly and fine structure of microtubule cytoskeleton could be observed. This method will play a guiding role in microtubule cytoskeleton investigation and observation of other plant cells.

关 键 词：植物细胞 微管 水稻根尖 免疫荧光组织化学染色 

分 类 号：S511[农业科学—作物学] Q245[生物学—细胞生物学]