造血干细胞移植预处理中内皮损伤与修复启动研究  

Endothelial injury and repair mechanism in allo-HSCT preconditioning

作　　者：鹿群先[1] 丁爽[1] 徐开林[1] 闫志凌[1] 贾路[1] 许世娟[1] 曾令宇[1] 

机构地区：[1]徐州医学院附属医院血液科实验室,徐州221002

出　　处：《国际输血及血液学杂志》2010年第2期116-119,共4页International Journal of Blood Transfusion and Hematology

基　　金：基金项目：国家自然科学基金项目（30572273）、江苏省自然科学基金（BK2007504）、江苏省徐州医学院院长人才基金（07KJZ25）和江苏省高校自然科学研究项目（06KJD310185）资助

摘　　要：目的 探索异基因造血干细胞移植预处理过程中，照射在摧毁造血系统的同时，对组织器官血管内皮的损伤及修复的启动。方法将20只6～8周龄雌性BALB／C小鼠随机分2组：对照组（n=10），为未照射小鼠；单纯照射组（n=10），小鼠经8．5Gy照射，剂量率为0．57Gy／min，距离为2．33m。照射后，两组分别取小鼠外周血，用流式细胞术分析两组小鼠外周血的内皮细胞（ECs，CD31＋CD133-CD45-）和内皮祖细胞（EPCs，CD31＋CD133＋CD4510w／-）细胞比例。照射后第5天HE切片观察两组小鼠组织结构损伤，电子显微镜观察损伤的超微结构变化。结果①照射组的ECs和EPCs细胞比例升高，第3～5天达峰值，且可维持4天高水平，与对照组相比差异有统计学意义（P〈0．05）。②HE切片中照射组的肝细胞有广泛炎症浸润，而内皮损伤不明显。电子显微镜下肝窦状间隙损伤，血小板聚集到暴露的胶原纤维上，ECs和基底膜的连接被破坏。结论异基因造血干细胞移植中预处理因素照射可引起内皮损伤，该损伤可引起EPCs动员至外周，参与内皮损伤的修复。Objective To explore endothelial damage of orgams and the repair mechanism in the allo- HSCT preconditioning-irradiation. Methods Twenty female mice aged six to eight-weeks were randomieed into two groups： control group（n= 10） and irradiation group（n= 10）. Mice in irradiation group were subjected to 8.5 GY irradiation （dose rate 0.57 GY/min, target distocnce 2.33 m）. Peripheral blood was collected at various time points to assay the circulating endothelial cells （ECs, CD31＋CD133-CD45-） and endothelial progenitor cells （EPCs, CD31 ＋ CD133 ＋ CD45low/- ） with flow cytometry. The Histological changes of endothelium were examined with phage contrast light microscopy at day 5 after irradiation. The integrity of endothelial vasculature was determined with transmission electron microscope （TEM） simultaneously. Results The numbers of ECs and EPCs cells increased gradually and reached the peak at day 3 or 5 in irradiation group （P〈0. 05） and remained high for 4 days. A systematic hydroncus of hepatocyte and inflammatory cell infiltration were observed under light microscopy in irradiation group. Endothelium injury was not visible. Aggregation of platelets on exposed collagen fiber was observed. Damage of the conjunction between ECs and hasilemma was found after irradiation with electron microscopy. Conclusions Irradiation could induce the injury of tissue, and drive the EPCs to circulation and trigger off the repair of injury in hematopoietic stem cells transplantation.

关 键 词：照射 内皮细胞 内皮祖细胞 动员 

分 类 号：R457[医药卫生—治疗学]