真武汤冲剂对压力负荷性心肌肥厚的影响  

Effects of Zhenwu decoction on rats with pressure-overloaded left ventricular myocardial hypertrophy

作　　者：谢志翔[1] 王舒茵[1] 梁子敬[1] 曾量波[1] 

机构地区：[1]广州市红十字会医院急诊科,广州510201

出　　处：《中华急诊医学杂志》2010年第4期385-388,共4页Chinese Journal of Emergency Medicine

基　　金：广东省自然科学基金（06022688）

摘　　要：目的观察真武汤冲剂对大鼠慢性压力负荷性心肌肥厚的影响。方法租用广州呼吸疾病研究所实验室，将SD大鼠随机（随机数字法）分为3组，假手术组8只，手术组和中药组各15只。手术组和中药组行腹主动脉缩窄术，制作心肌肥厚模型。16周行心脏超声，确定术后心肌肥厚，中药组加用真武汤混合饲料喂养，24周时用高频超声测定各组心脏质量、结构和功能，以及心肌组织Masson染色，测定总胶原容积百分比（CVF-T）和无冠状动脉小血管视野胶原容积百分比（CVF—NV）。各组数据采用SNK法进行两两比较。结果手术组心脏质量增加，以左室增加为明显（P〈0．05），心脏增大，以舒张期室间隔（IVSd）和左室后壁（LVPWd）为明显（P〈0．01），心功能明显降低（P〈0．05）；中药组相对于手术组，心脏质量减轻（．P〈0．01），体积缩小，以1VSd和收缩期左室后壁（LVPWs）为明显（P〈0．01），心功能改善（P〈0．05）。病理分析手术组间质和胶原增牛明显，中药组有不同程度改善，CVF-T和CVF-NV较手术组组明显减少（P〈0．01），与假手术组相比，差异无统计学意义（P〉0．05）。结论真武汤冲剂能够减少压力负荷性大鼠的心脏质量、体积，改善心功能，抑制胶原纤维增生，逆转心肌肥厚。Objective To investigate the effects of Zhenwu decoction （a herbal preparation made in a form of instant granules readily soluble into decoction to administer） on rats with chronic pressure-overloaded left ventricular hypertrophy. Method All the male SD rats were randomly（random number） divided into 3 groups： sham operation group （ n = 8）, operation group （ n = 15） and herbal medicine group （ n = 15）. The model of myocardial hypertrophy was made by gradually constricting the abdominal aorta. After 16 weeks, cardiac uhrasonography was used in rats of all groups to ascertain the post-operational left ventricular （LV） hypertrophy established. And the Zhenwu decoction was added in the herbal medicine group in a dose of 12 g/kg mixed in feedstuff fed for 8 weeks. After 24 weeks, the weight and structure as well as function of the heart of rats in each group were measured by high-frequency ultrasonography, and Massion stain was performed on cardiac muscles. Meanwhile, both the total collagen volume fraction （CVF-T） and non-coronary vessel collagen volume fraction （CVF-NV） were analyzed. Results There was an increase in the weight of the heart with a predominance of the left ventriele in the operation group （ P 〈 0.05）. The heart was enlarged with diastolic interventricular septum thickness （IVSt） and left ventricular posterior wall thickness （LVPWt） increased predominantly （ P 〈 0.01 ）. There was a significant decrease in cardiac function （ P 〈 0.05）. In herbal medicine group, both the weight and volume of heart were lower than those in operation group with predominance of IVSt and systolic left ventricular wall （P 〈 0.01 both）. And the cardiac function of rats in herbal group was better （P 〈 0.05）. Interstitial and collagen hyperplasia increased significantly in the rats of operation group found by pathological analysis. In herbal medicine group, the pathological changes were blunted in varied magnitude with significant decrease in CVF-T and CVF-N

关 键 词：真武汤 心力衰竭 心室重构 心肌肥厚 压力负荷 Massion染色 心肌胶原纤维 腹主动脉缩窄 

分 类 号：R285.5[医药卫生—中药学]