黄芪及缬沙坦对肾型高血压大鼠心脏血管紧张素转化酶2表达的影响  

Effects of radix astragali on expression of angiotensin-converting enzyme 2 in heart of 2 kidney 1 chip hypertensive rats

作　　者：任红梅[1] 余静[2] 张缤[2] 

机构地区：[1]宁夏回族自治区人民医院心内科,银川750024 [2]兰州大学第二附属医院心内科

出　　处：《临床心血管病杂志》2010年第3期216-219,共4页Journal of Clinical Cardiology

摘　　要：目的：探讨两肾一夹高血压大鼠心脏血管紧张素转化酶2（Angiotensin-Converting Enzyme2,ACE2）mRNA、蛋白表达水平及甘肃定西黄芪（Astragalus in Gansu Dingxi,Ast）和缬沙坦（Valsartan,Val）对其表达的影响。方法：健康雄性Wistar大鼠,体重180~200g,构建两肾一夹KIC高血压模型。术后随机分为5组,假手术组;②2KIC组;③Val＋2KIC组;④Ast＋2KIC组（低剂量）;⑤Ast＋2KIC组（高剂量）。在观察期末,分别测定各组大鼠体重、心脏湿重,计算出心脏重量/体重;光镜下观察心肌结构的变化;RT-PCR检测各组心肌组织ACE2mRNA,免疫组化测定其蛋白表达。结果：与假手术组比较,2KIC组、Val＋2KIC、Ast＋2KIC组（低剂量）、Ast＋2KIC组（高剂量）心脏重量/体重均升高（P〈0.05）;②与假手术组比较,2KIC组ACE2mRNA、蛋白表达显著减少;与2KIC组比较,Val＋2KIC组及Ast＋2KIC组（高剂量）经8周治疗后,ACE2mRNA、蛋白表达量增加（P〈0.05）。结论：高血压大鼠心脏ACE2mRNA、蛋白表达减少,Ast＋2KIC组（高剂量）及Val＋2KIC组可上调高血压大鼠心脏ACE2水平,此作用可能说明甘肃定西黄芪具有调节肾素-血管紧张素系统的作用。Objective：To investigate the expression of ACE2 MRNA and angiotensin-converting enzyme 2（ACE2）in the heart of 2kic hypertensive rats and the effects of（Valsartan,Val）and Astragalus in Gansu Dingxi（Ast）.Methods：Healthy male wistar rats,weight 180-200 g,are randomly divided into 5 groups after operation.There are 9 rats in each group.Sham operated group were served as control group,2KIC group,2KIC＋val group,2KIC＋Ast group（low dose）and 2KIC＋Ast group（high dose）.The following parameters were examined：body weight（BW）,heart weight（HW）.The ratio of HW/BW was calculated;ACE2 mRNA and protein were tested by RT-PCR and immunohistochemistry,respectively at the same period.Results：①compared with the control group,the ratio of HW/BW were all increased in 2KIC group,2KIC ＋Val group,2KIC ＋Ast group（low dose）and 2KIC ＋Ast group（high dose）（P0.05）,②ACE2 in the heart was significantly down-regulated in the 2Kic group compared with the control group.After 8 weeks treatment with astragalus（high dose）and valsartan,compared with the 2KIC group,ACE2 mRNA and protein was up-regulated in 2KIC ＋Ast（high dose）and 2KIC ＋Val group.Conclusion：ACE2 MRNA and protein are down-regulated in hypertension-induced in rats and valsartan and astragalus are increased ACE2 expression.It suggests that astragalus in gansu dingxi have function of regularing Renin Angiotensin System.

关 键 词：高血压 黄芪 大鼠 血管紧张素转化酶2 缬沙坦 

分 类 号：R373[医药卫生—病原生物学]