复方芪麝片对直立大鼠腰椎成骨作用的观察  

Effect of FU FANG Qi She Pian on bone formation of upright posture rats′ vertebre

作　　者：卞琴[1] 梁倩倩[1] 侯炜[1] 卢盛[1] 赵永见[1] 施杞[1] 王拥军[1] 

机构地区：[1]上海中医药大学附属龙华医院上海中医药大学脊柱病研究所

出　　处：《中国脊柱脊髓杂志》2010年第4期311-316,共6页Chinese Journal of Spine and Spinal Cord

基　　金：国家杰出青年科学基金(No.30625043);国家自然科学基金(No.30572398);上海市优秀学科带头人计划(No.08XD1404000)

摘　　要：目的:观察复方芪麝片(FFQSP)对直立体位大鼠腰椎骨形成的作用,探讨可能的作用机制。方法:90只SD大鼠随机分为三大组,正常组不进行处理,普通饲养笼喂养;模型组和FFQSP组,建立长期直立体位大鼠模型。每大组再随机分为5、7和9个月组,FFQSP组分别在造模后4、6、8个月开始应用FFQSP(将片剂研磨溶于生理盐水)灌胃,连续灌胃1个月;模型组和正常组用同量生理盐水灌胃1个月。所有动物于相应时间点取整个腰椎进行检测,行L5椎体藏红-固绿染色及Ⅰ型胶原(typeⅠcollegan,ColⅠ)免疫组织化学染色,余下腰椎椎体应用实时荧光定量聚合酶链反应(real time reverse transcription polymerass chain reaction,real time RT-PCR)技术检测Ⅰ型胶原α2(typeⅠcolleganα2,Col1α2)、转化生长因子beta1(transform growth factor beta1,TGF-β1)和runt相关转录因子2(runt-related transcription factor2,Runx2)基因的表达。结果:藏红染色显示,与正常组比较,三个时间点模型组及FFQSP组椎体骨小梁均增加,FFQSP组在5个月和7个月时较模型组略有增加;在9个月时较模型组略有减少。ColⅠ免疫组织化学染色显示三个时期模型组骨小梁阳性染色数目较正常组均增加,FFQSP组骨小梁阳性染色范围较正常组、模型组均增加。Real time RT-PCR示,与模型组比,5个月FFQSP组Col1α2、TGF-β1基因表达显著下调(P<0.05,P<0.01),与正常组比,仅上调Runx2的表达(P<0.01)。7个月FFQSP组Col1α2、TGF-β1、Runx2基因表达较正常组和模型组都显著上调。9个月FFQSP组与模型组比较,Col1α2基因表达显著下调(P<0.05),而TGF-β1和Runx2基因表达均显著上调(P<0.05);与正常组比,三个基因表达均显著上调(P<0.01)。结论:复方芪麝片对直立大鼠的椎体成骨有增强作用,以中后期(7个月、9个月)效应显著,其疗效可能与TGF-β1基因水平的上调有关。Objective：To investigate the effect and potential mechanism of Fu Fang Qi She Pian（FFQSP） on upright rats′ lumbar vertebral bone formation.Method：Ninety SD rats were randomly divided into three groups.The rats in the normal control group were raised in normal cage with no intervention.The rats in untreated and FFQSP groups were induced to upright posture.Every group was randomly divided into three subgroups：5 months,7 months and 9 months group.Rats in FFQSP groups were gastroentericly administrated with FFQSP at 4,6,8 months for one month after surgery while those in model and control group were treated with normal saline.Ten rats were sacrificed at 5,7 and 9 months after surgery and all lumbar vertebre were harvested for detection.Safranine O/fast green staining and Col Ⅰ immunohistostaining were performed on L5 vertebrae.Col1α2、TGF-β1 and Runx2 mRNA expression in other vertebre were detected by real time reverse transcription polymerass chain reaction（RT-PCR）.Result：Safranin O/Fast green staining showed bone trabecular density increased in untreated groups.BV/TV rate raised in 9 months of untreated group compared to normal control group,and it went up in 5,7 months of FFQSP groups compared to untreated groups.Trabecular numbers increased in 5 and 9 months of untreated groups compared to those in the normal group when it increased in 5 and 7 months of FFQSP groups compared to those in untreated groups.Immunohistostaining showed in creased numbers of bone trabeculae with Col Ⅰ positive staining after surgery,which extended in FFQSP groups compared to that in control and model groups at each time points.Real time RT-PCR showed Col1α2 and TGF-β1 mRNA expression significantly down-regulated in FFQSP group compared to those in model group at 5 months（P〈0.05,P〈0.01）.However,only Runx2 expression was up-regulated compared with normal group（P〈0.01）.Col1α2,TGF-β1 and Runx2 gene expression increased in FFQSP groups at 7 months compared with those in control and model groups at t

关 键 词：复方芪麝片 腰椎 成骨 气血 

分 类 号：R965[医药卫生—药理学] R681.5[医药卫生—药学]