重庆南瓜病毒病病原ELISA检测及CMV变异分析  

ELISA Detection of Infectious Pathogenic Viruses and Variation of CMV from Squash in Chongqing

作　　者：青玲[1,2] 包凌云[1] 周常勇[1,2] 杨水英[1] 孙现超[1] 

机构地区：[1]西南大学植物保护学院,重庆400716 [2]中国农科院柑橘研究所,重庆400712

出　　处：《园艺学报》2010年第3期405-412,共8页Acta Horticulturae Sinica

基　　金：科技部科技基础性工作专项(2006FY111000);西南大学博士后基金项目(207001)

摘　　要：利用7种植物病毒的抗体,对采自重庆7个区县的67份南瓜病毒病样品进行了抗原直接包被酶联免疫吸附分析(ACP-ELISA)检测。检测结果表明,在这67份样品中,至少59份感染了所要检测病毒,其中,感染黄瓜花叶病毒(Cucumber mosaic virus,CMV)、烟草花叶病毒(Tobacco mosaic virus,TMV)、番茄花叶病毒(Tomato mosaic virus,ToMV)、芜菁花叶病毒(Turnip mosaic virus,TuMV)、蚕豆萎蔫病毒2号(Broad bean wilt virus 2,BBWV-2)、马铃薯Y病毒(Potato virus Y,PVY)和马铃薯X病毒(Potato virus X,PVX)的样品分别为80.60%、77.61%、74.63%、82.09%、76.12%、83.58%和55.22%。在检测呈阳性的59份样品中有93.22%的样品受到2种以上病毒的复合侵染,而受7种病毒复合侵染的样品高达60.00%,表明在南瓜上病毒复合侵染现象相当严重。进一步对54份受CMV侵染的南瓜样品检测发现,CMV亚组Ⅰ(CMVⅠ)病毒侵染的样品有49份,占90.74%,CMV亚组Ⅱ(CMVⅡ)病毒侵染的样品为11份,占20.37%;其中6份(11.11%)样品检测到CMVⅠ和CMVⅡ的复合侵染。以免疫捕获反转录PCR(IC-RT-PCR)扩增了一个CMVⅠ分离物(CMV-CQ)近全长CP基因并进行序列分析,结果证实其属于CMV亚组Ⅰ型,其核苷酸序列与国内CMVⅠ型赤豆分离物(CMV-RB)的同源性最高,为98.1%。The 67 squash samples with viral symptoms collected from seven squash-growing counties in Chongqing were detected by antigen–coated plate（ACP）-ELISA with antibodies against seven plant viruses.The results showed that at least 59 samples with in these 67 squash samples were infected by the viruses,of which 80.60%、77.61%、70.15%、82.09%、76.12%、83.58% and 55.22% of the samples were infected by Cucumber mosaic virus（CMV）,Tobacco mosaic virus（TMV）,Tomato mosaic virus（ToMV）,Turnip mosaic virus（TuMV）,Broad bean wilt virus 2（BBWV-2）,Potato virus Y（PVY） and Potato virus X（PVX）,respectively.Among 59 positive samples,93.22% of the samples were mix-infected with more than two viruses,and about 60% samples were mix-infected by the above seven viruses,indicating the mix-infections of viruses on squash were severely.Moreover,the results showed that among 54 samples infected by CMV,49（90.74%）samples were infected by CMV subgroupⅠ（CMVⅠ）and 12（22.22%）samples by CMV subgroup.Comparison of the nucleotide sequences showed that it has the highest identity（98.1%） with the previously reported CMVⅠisolate infecting Phaseolus angularis（CMV-RB）from China.

关 键 词：南瓜病毒病 ELISA检测 黄瓜花叶病毒亚组 外壳蛋白基因 序列变异 

分 类 号：S642.1[农业科学—蔬菜学] S436[农业科学—园艺学]