朝鲜白头翁的组织培养与快繁技术  

Studies on Tissue Culture and Rapid Propagation of Pulsatilla koreana Nakai

作　　者：廉玉姬[1] 赵小梅[1] 林光哲[1] 

机构地区：[1]山东省临沂师范学院生命科学学院,山东临沂276005

出　　处：《园艺学报》2010年第3期491-498,共8页Acta Horticulturae Sinica

基　　金：山东省自然科学基金项目(Y2008D17)

摘　　要：以朝鲜白头翁的叶片、叶柄和花梗为材料,研究了细胞分裂素zeatin、kinetin、6-BA和生长素IAA组合对诱导不定芽的影响;以不定芽为材料研究了植物生长调节剂对不定芽的增殖和生根的影响;研究了不同基质配比对组培苗移栽成活的影响。结果表明:1)叶柄和花梗接种在MS+0.5mg·L-1zeatin+0.05mg·L-1IAA培养基培养6周后不定芽再生率为100%。2)不定芽切成小块或单株带类似愈伤的组织块转到MS+0.5mg·L-1 zeatin+0.05mg·L-1IAA或MS+1.0mg·L-16-BA+0.05mg·L-1 IAA的培养基时不定芽的增殖与生长良好。3)在增殖培养基中长至3cm以上的小植株转入到1/2MS+1.5mg·L-1NAA的生根培养基中培养42d后,生根率高达93%,平均根长12.3cm,每株平均根数11条,且可缩短生根时间。4)组培苗移栽到东北山土和珍珠岩比例为2︰1的混合基质时,成活率高达66.7%。Leaf,petiole and pedicle explants of Pulsatilla koreana were cultured on MS medium supplemented with various concentrations of zeatin,kinetin or 6-BA combined with 0.05 mg·L^-1 IAA.After 6 weeks of culture,effects of cytokinin on adventitious shoot formation from explants were investigated.The highest frequency of shoot formation was obtained when petiole and pedicle explants were cultured on MS＋0.5 mg·L^-1 zeatin＋0.05 mg·L^-1 IAA.Regenerated shoot were cut as several cluster or single plant with callus like tissue,and then transferred on MS＋0.5 mg·L-1 zeatin＋0.05mg·L^-1 IAA or MS＋1.0 mg·L^-1 6-BA＋0.05 mg·L^-1 IAA for shoot propagation and growth.When shoot reached more than 3 cm in length,they were transferred on to root induction medium.93 percent of rooting ratio was observed in 1/2 MS＋1.5 mg·L^-1 NAA,after 42 days of culture.The rooted plantlets were acclimatized on mixture of North east mountain soil and perlite（2：1）.

关 键 词：朝鲜白头翁 不定芽 细胞分裂素 生根 移栽 

分 类 号：S567[农业科学—中草药栽培]