雄性受体小鼠性腺对移植卵巢卵母细胞生长发育的影响  

Effect of Gonad of Male Recipient on the Development of Allografted Ovaries and Their Oocytes

作　　者：彭南妮[1,2] 薛立群[1] 袁安文[1] 凌理军[1] 吴珊[1] 

机构地区：[1]湖南农业大学动物医学院与动物科技学院,长沙410128 [2]深圳市罗湖区人民医院生殖医学中心,深圳518000

出　　处：《中国实验动物学报》2010年第2期142-144,I0008,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：湖南省重点科技项目(编号:01NKY1002-01);湖南省科技厅计划项目(编号:2007TP4024)

摘　　要：目的探索雄性受体性腺对移植卵巢卵母细胞生长发育的影响。方法将1日龄小鼠卵巢移植入成年雄鼠肾囊下,将雄性受体小鼠分为性腺摘除组和性腺在位组,于21d回收移植卵巢,以评价雄性小鼠性腺对新生小鼠卵巢移植体卵母细胞生长发育的影响。结果移植后21d,性腺摘除组和性腺在位组卵巢回收率分别为80.0%和92.9%,移植体生长增大;每个卵巢平均回收卵母细胞数分别为(30.4±4.3)和(42.4±11.1)个,两者差异不显著(P>0.05);性腺摘除组回收的均为GV期卵母细胞,性腺在位组大部分为GV期卵母细胞,有的达到MII期。结论雄性受体小鼠能够支持移植卵巢卵母细胞的生长发育,雄性受体的性腺对其影响不明显。Objective To investigate the effect of gonad of male recipient on development of neonatal ovary allograft and its oocytes.Methods The ovaries from one-day-old mice were transplanted underneath the kidney capsule of male recipient with or without gonad.The ovarian grafts were collected at 21 days after transplantation and examined by histopathology.Results It was found that the ovarian grafts increased in size,the recovery rate and average number of oocytes collected from each graft in both groups were 92.9% and 80.0% ,42.4 ± 11.1 and 30.4 ± 4.3,respectively.The difference was not significant（P 0.05）.The oocytes collected from the grafts in castrated male recipients were at the GV stage,and except a few at metaphase II stage most of the oocytes in intact male recipients developed at GV stage.Conclusion The results indicate that the grafted ovarian oocytes can develop in male recipient mice and the gonad of male recipient has no significant effects on oocyte development.

关 键 词：小鼠 移植 性腺 卵巢 卵母细胞 

分 类 号：Q813.3[生物学—生物工程]