丙型肝炎病毒核心抗原检测的临床应用探讨  

Clinical significance of testing hepatitis C virus core antigen

作　　者：朱鸿[1] 张韬[1] 范存琳[1] 肖晓光[1] 张凤华[1] 

机构地区：[1]大连医科大学附属第一医院检验科,辽宁大连116011

出　　处：《大连医科大学学报》2010年第2期211-212,218,共3页Journal of Dalian Medical University

摘　　要：[目的]探讨HCV核心抗原在HCV感染诊断和治疗中的作用。[方法]对173例HCV感染患者血清和82例健康对照者血清,使用ELISA法分别检测抗-HCV、总的HCV核心抗原,使用荧光定量RT-PCR检测HCV RNA。[结果]173例HCV感染者抗-HCV均为阳性。HCV RNA阳性率为49.7%(86/173);HCV核心抗原阳性率为36.4%(63/173)。二者差异有统计学意义(P<0.05)。86例HCV RNA阳性标本中,HCV核心抗原阳性60例(69.8%);HCV核心抗原阴性26例(30.2%)。82例健康对照者抗-HCV、HCV核心抗原、HCV RNA均为阴性。[结论]在没有HCV RNA检测资格的实验室,HCV核心抗原检测可作为病毒存在和复制的一个指标,作为抗-HCV检测的补充试验。[Objective]To investigate the significance of serum total HCV core antigen（HCV-cAg） in the HCV infection and treatment.[Methods]One hundred and seventy-three serum specimens of HCV infection patients were examined for HCV cAg and anti-HCV antibody with ELISA,for HCV RNA with real-time RT-PCR.[Results]In 173 anti-HCV positive specimens,the positive rate of HCV RNA was 49.7%（86/173） and the positive rate of HCV-cAg was 36.4%（63/173）.The positive rate of HCV RNA was higher than that of HCV-cAg（P〈0.05）.In 86 HCV RNA positive specimens,the positive rate of HCV-cAg was 69.8%（60/86）,the negative rate was 30.2%（26/86）.The control was negative.[Conclusions]In laboratory where it is impossible for HCV RNA measurement by using RT-PCR,HCV-cAg assay in serum will be very useful for the clinical diagnosis of hepatitis C and the detection of HCV.

关 键 词：丙型肝炎病毒 核心抗原 抗体 RNA 

分 类 号：R446.6[医药卫生—诊断学]