高脂血症小鼠模型耳蜗中核因子-κB p65的表达  

Expression of nuclear factor-kappa B p65 in hyperlipemia model of mice

作　　者：杜政德[1] 余青松[1] 向一[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉430022 [2]华中科技大学同济医学院药学院

出　　处：《临床耳鼻咽喉头颈外科杂志》2010年第7期289-292,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨高脂血症对小鼠耳蜗的影响。方法:20只昆明种小鼠随机平均分为2组,其中1组建立高脂血症模型,另1组作为对照,6周后用多克隆兔抗NF-κB p65免疫组织化学染色,观察NF-κB p65在高脂血症小鼠耳蜗中的表达,并检测小鼠ABR反应阈。结果:在高脂血症小鼠耳蜗中,NF-κB p65主要定位在Corti器、盖膜、血管纹、螺旋韧带、螺旋神经节及其神经纤维,高脂血症小鼠耳蜗NF-κB p65的免疫反应与对照组比较显著增强。高脂血症模型组小鼠ABR反应阈与对照组比较明显增高,差异有统计学意义(P<0.01)。结论:高脂血症可使小鼠耳蜗NF-κB p65表达增强,ABR反应阈增高,表明高脂血症可引起小鼠听力损害。objective：To study the expression of nuclear factor kappa B p65 in hyperlipemia model of mice,and the relationship between hyperlipemia and deaf.Method：Twenty mice were divided into two group.The hyperlipemia diet group was established in ten mice,and the normal diet group was served as normal control.Six weeks later,immunohistostaining was used to detected the express of NF-κB p65 in all mouse cochlear. ABR threshold was obtained from both normal group and hyperlipemia group.Result：Immunoreactivity NF-κB p65 in mouse cochlea of hyperlipemia was localized in the organ of Corti, tectorial membrane,stria vascularis,spiral ligament,spiral ganglion and nerve fibers. The NF-κB p65 expression was markedly increased in mouse cochlea of hyperlipemia ABR threshold was significant differencel between hyperlipemia group mice and control group mice（P〈0.01）.Conclusion：The expression of NF-κB p65 in mouse cochlea can be induced by hyperlipemia.And ABR threshold increased in hyperlipemia group mice. This shows that hyperlipidemia can damage acouesthesia of mice.

关 键 词：高脂血症 NF-κB P65 小鼠耳蜗 ABR反应阈 免疫组织化学 

分 类 号：R589.2[医药卫生—内分泌]