人工关节感染患者BACTEC Plus/F瓶和普通细菌培养比较  

Bacteria culture in BACTEC Plus/F bottles for detection of pathogens in prosthetic-joint infections

作　　者：邹国友[1] 张先龙[1] 沈灏[1] 蒋垚[1] 

机构地区：[1]上海交通大学医学院附属第六人民医院骨科人工关节外科,上海200233

出　　处：《临床骨科杂志》2010年第2期145-147,共3页Journal of Clinical Orthopaedics

摘　　要：目的评估BACTEC Plus/F瓶在人工关节假体感染的患者关节液和组织中细菌培养的优越性。方法58例临床诊断为人工关节感染的患者,手术中抽取关节液和切取假体周围软组织,进行细菌常规培养和BACTEC Plus/F瓶增殖培养。手术中快速冰冻切片病理检查。结果58例病理检查均证实有炎症反应,支持临床诊断。组织匀浆检出率:BACTEC Plus/F培养为79.3%,常规培养为50%,两者差异有统计学意义(P=0.005)。关节液细菌检出率:BACTEC Plus/F为74.1%,常规培养为45.1%,两者差异有统计学意义(P=0.003)。污染率两者差异亦有统计学意义(P=0.041)。结论BACTEC Plus/F瓶人工关节假体感染的患者培养细菌检出率更高,应该在人工关节感染患者中常规应用。Objective To compare the detection rates of bacteria by culture in BACTEC Plus/F bottles and agar plate.Methods Periprosthetic tissue specimens and synovial fluid were obtained during revision from 58 patients with clinical diagnosed prosthetic-joint infections.Samples were examined by microbiological culture over a 14-day period,and the tissues were subjected to quick frozen section pathologic examination.Results For periprosthetic tissue samples,the sensitivities of BACTEC Plus/F bottle and conventional agar plate cultures for the diagnosis of prosthetic-joint infections were 79.3% and 50%（P=0.005）;For synovial fluid samples,the sensitivities of BACTEC Plus/F bottle and conventional agar plate cultures were 74.1% and 45.1%（P=0.003）.Overall,culture in BACTEC Plus/F bottles detected significantly fewer contaminants than culture on agar plates（1 versus 6 contaminants,P=0.041）.Conclusions These results indicate a superior performance of BACTEC Plus/F bottle cultures over conventional agar plate cultures for the detection of pathogens in patients with prosthetic-joint infections.

关 键 词：人工关节感染 关节成形术 置换 细菌培养 

分 类 号：R684[医药卫生—骨科学] R446.51[医药卫生—外科学]