苦参素对大鼠急性脊髓损伤后热休克蛋白47表达的影响  

Effect of oxymatrine on heat shock protein 47 expression in rat acute spinal cord injury

作　　者：何玉宝[1] 杨晨[2] 杨有庚[1] 任宪盛[1] 

机构地区：[1]吉林大学第二临床医学院骨科,吉林长春130041 [2]吉林大学第一临床医学院骨关节科,吉林长春130041

出　　处：《临床骨科杂志》2010年第2期211-214,共4页Journal of Clinical Orthopaedics

摘　　要：目的探讨苦参素治疗大鼠急性脊髓损伤(SCI)的机制。方法用30只Wistar大鼠制作钳夹型SCI模型,随机分成正常组、假手术组、SCI组、对照组和治疗组,在SCI后3d及每周评价BBB评分,并在第8周用免疫组化方法分析脊髓中热休克蛋白47(HSP47)的表达情况。结果正常组和假手术组Wistar大鼠脊髓组织低表达胶质细胞酸性蛋白(GFAP)和HSP47,高表达神经丝蛋白(NF),在SCI后GFAP和HSP47表达明显提高(P<0.05),NF表达明显降低(P<0.05)。苦参素治疗后,GFAP和HSP47表达降低(P<0.05),NF表达升高(P<0.05)。结论苦参素对Wistar大鼠SCI具有治疗作用,很可能通过抑制HSP47的表达发挥治疗作用。Objective To discuss the therapeutic mechanism of oxymatrine in rat acute spinal cord injury（SCI）.Methods A model of clip-compression injury of spinal cord in rats was used.30 Wistar rats were divided to normal group,sham operation group,SCI group,control group and therapeutic group randomly.Basso,Beattie,Bresnahan（BBB） Locomotor Rating Scale were assessed at third day and every week after SCI.They were sacrificed to study the expression level of heat shock protein 47（HSP47） using immunohistochemistry technique at eighth week.Results There was a lower expression level of glial fibrillary acidic protein（GFAP） and HSP47 in normal group and sham operation group,while the level of neurofilament protein（NF） was higher.But,the expression level of GFAP and HSP47 after SCI was higher（P0.05）,while the level of NF was lower（P0.05）.The expression level of GFAP and HSP47 in therapeutic group was lower（P0.05）,while the level of NF was higher（P0.05）.Conclusions SCI in Wistar rats can be treated with oxymatrine.It probably suppresses the expression of HSP47.

关 键 词：脊髓损伤 HSP47热休克蛋白质类 钳夹型SCI模型 苦参素 

分 类 号：R681.5[医药卫生—骨科学] R341[医药卫生—外科学]