成人脂肪源间充质干细胞在小鼠体内具有血液血管干细胞特性  

Hemangioblastic characteristics of human adipose tissue-derived adult stem cells in vivo

作　　者：马慧珍[1] 李宁[2] 宋永平[2] 曹莹[2] 董子明[1] 房佰俊[1,2] 

机构地区：[1]郑州大学基础医学院,河南郑州450000 [2]河南省肿瘤医院,河南郑州450003

出　　处：《中国病理生理杂志》2010年第4期656-661,共6页Chinese Journal of Pathophysiology

基　　金：河南省杰出青年基金资助项目(No.0612000900)

摘　　要：目的:观察成人脂肪源Flk1+ CD31- CD34-细胞在小鼠体内是否具有血液血管干细胞的特性。方法:将男性成人脂肪源Flk1+ CD31- CD34-细胞经尾静脉输入6-8周龄、接受亚致死剂量射线照射的非肥胖型糖尿病及严重联合免疫缺陷(NOD/SCID)小鼠体内(每只输入剂量为105,悬于0.4m LRPMI-1640培养基中),对照组接受同等剂量的RPMI-1640培养基中。2个月后处死小鼠。用聚合酶链式反应、流式细胞术、三色荧光法及荧光原位杂交法检测脂肪源Flk1+ CD31- CD34-细胞在实验小鼠骨髓及消化系统内的分化情况。结果:脂肪源Flk1+ CD31- CD34-细胞在单细胞水平上在NOD/SCID小鼠体内可分化为内皮细胞和造血细胞。结论:脂肪源Flk1+ CD31- CD34-细胞在体内具有血液血管干细胞的特性,这类细胞有可能作为种子细胞用以治疗血液和血管性疾病。AIM：To investigate whether Flk1^＋CD31^-CD34^- cells isolated from human adult adipose tissue have characteristics of hemangioblasts in vivo. METHODS： After sublethally irradiated （300cGy） with a caesium source,the female non-obese diabetic/severe combined immunodeficient （NOD/SCID） mice were injected with human adipose tissue-derived Flk1^＋CD31^-CD34^- cells （105 cells per mouse） via tail vain with 0.4 mL Roswell Park Memorial Institute medium （RPMI-1640）. The control mice received the same volume of RPMI-1640 medium. All mice were killed 2 months after transplantation for further study. The differentiation potential of Flk1^＋CD31^-CD34^- cells was assessed in bone marrow and gastrointestinal tract by the methods of flow cytometry,RT-PCR,FISH,and triple-color immunofluorescence. RESULTS： Flk1^＋CD31^-CD34^- human adipose tissue-derived adult stem cells differentiated into endothelial cells and hematopoietic cells at the single-cell level in vivo. CONCLUSION： Human adult adipose tissue-derived Flk1^＋CD31^-CD34^- cells bear characteristics of hemangioblast in vivo and may have potential application for the treatment of hematopoietic and vascular diseases.

关 键 词：造血干细胞 内皮细胞 脂肪组织 

分 类 号：R331.1[医药卫生—人体生理学]