积雪草提取物的抗氧化及免疫调节作用研究  

Antioxidant and immunoregulatory effects of Centella asiatica extracts

作　　者：贺惠娟[1,2] 李菁[1] 朱伟杰[3] 卢丽华[4] 

机构地区：[1]暨南大学医学院病理生理学系,广东广州510632 [2]湖北中医学院 [3]暨南大学生殖免疫研究中心,广东广州510632 [4]北京大学深圳医院,广东深圳518036

出　　处：《中国病理生理杂志》2010年第4期771-776,共6页Chinese Journal of Pathophysiology

基　　金：国家中医药管理局三级实验室开放课题基金资助项目

摘　　要：目的:观察积雪草提取物的抗氧化作用及其对小鼠脾淋巴细胞增殖的影响。方法:采用乙醇浸提及极性萃取分离提取积雪草活性物质,用福林法测定提取物多酚含量并观察其抑制1,1-二苯基-2-苦基苯肼(DPPH)自由基、羟自由基及细胞内活性氧(ROS)的能力。应用脾淋巴细胞增殖模型,观察各相提取物对淋巴细胞增殖率的影响。结果:(1)积雪草含有丰富多酚类物质,各相提取物多酚含量依次为:乙酸乙酯相>正丁醇相>水相>石油醚相。(2)积雪草各相提取物均具有抑制DPPH自由基、羟自由基的能力,其中乙酸乙酯相作用最为显著。(3)积雪草乙酸乙酯相对氧化应激状态下脾淋巴细胞内ROS的生成有显著抑制作用。(4)积雪草乙酸乙酯相提取物对脾淋巴细胞增殖有抑制作用。(5)经初步鉴定,积雪草乙酸乙酯相提取物为多酚家族中的黄酮类物质。结论:积雪草具有较强的抗氧化能力,其主要活性部位在乙酸乙酯相,且主要成份为黄酮,并具有一定的免疫调节功能。AIM：To determine the antioxidant and the immunoregulatory effects of Centella asiatica extracts. METHODS： Centella asiatica was extracted with alcohol and different organic solvent. The content of polyphenol was determined by Folin-ciocalteau method. The efficacy of the extracts to scavenge the hydroxy radical （OH·）,1,1-diphenyl-2-picryl-hydrazyl radical （DPPH·） and intra-cellular reactive oxygen species （ROS） was measured. Lymphocyte proliferation was assessed to observe the influence of Centella asiatica extracts. RESULTS： （1） Centella asiatica was found to have abundant polyphenol extrated from different phases and in a descending order as follows： ethyl acetate extracts〉n-butanol extracts〉water extracts〉ligarine extracts. （2） Extracts of Centella asiatica exhibited the scavenging efficacy of OH· and DPPH· free radicals,in which the acetic ether extracts showed the significant effect. （3） The acetic ether extracts had significant ability to inhibit the generation of ROS in stimulated lymphocytes. （4） The acetic ether extracts suppressed the lymphocyte proliferation. （5） The active ingredient was identified as flavone. CONCLUSION： Flavones in the Centella asiatica posseses antioxidant activity and effectively inhibits lymphocyte proliferation,showing ability of immunosuppre-ssion.

关 键 词：积雪草 自由基 抗氧化 脾淋巴细胞增殖 免疫抑制 

分 类 号：R961[医药卫生—药理学]